Acyl-phosphates initiate membrane phospholipid synthesis in gram-positive pathogens
Acyl-phosphates initiate membrane phospholipid synthesis in gram-positive pathogens
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DOI：
10.1016/j.molcel.2006.06.030
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发表时间：
2006-09-01
期刊：
影响因子：
16
通讯作者：
Rock, Charles O.
中科院分区：
文献类型：
--
作者：
Lu, Ying-Jie;Zhang, Yong-Mei;Rock, Charles O.
目前尚不清楚革兰氏阳性细菌病原体如何进行甘油-3-磷酸（G3 P）酰化，这是磷脂酸形成的第一步，磷脂酸是膜磷脂合成的关键中间体。在大肠杆菌中，G3 P的1-位的酰化由PlsB进行;然而，大多数细菌缺乏plsB基因，并且在其他细菌中它不是必需的。我们描述了一个两步的途径，利用一个新的脂肪酸中间体开始磷脂的形成。首先，PlsX通过催化酰基-酰基载体蛋白合成脂肪酰基-磷酸产生独特的活化脂肪酸，然后PlsY将脂肪酸从酰基-磷酸转移到G3 P的1-位。PlsX/Y途径定义了用于启动细菌中磷脂形成的最广泛分布的途径，并且代表了用于开发抗菌治疗剂的新靶点。
It is not known how Gram-positive bacterial pathogens carry out glycerol-3-phosphate (G3P) acylation, which is the first step in the formation of phosphatidic acid, the key intermediate in membrane phospholipid synthesis. In Escherichia coli, acylation of the 1-position of G3P is carried out by PlsB; however, the majority of bacteria lack a plsB gene and in others it is not essential. We describe a two-step pathway that utilizes a new fatty acid intermediate for the initiation of phospholipid formation. First, PlsX produces a unique activated fatty acid by catalyzing the synthesis of fatty acyl-phosphate from acyl-acyl carrier protein, and then PlsY transfers the fatty acid from acyl-phosphate to the 1-position of G3P. The PlsX/Y pathway defines the most widely distributed pathway for the initiation of phospholipid formation in bacteria and represents a new target for the development of antibacterial therapeutics.