A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo
A near-infrared reversible fluorescent probe for real-time imaging of redox status changes in vivo
复制标题
一种近红外可逆荧光探针，用于体内氧化还原状态变化的实时成像
DOI：
10.1039/c2sc22076h
复制
发表时间：
2013-01-01
期刊：
影响因子：
8.4
通讯作者：
Tang, Bo
中科院分区：
文献类型：
--
作者：
Xu, Kehua;Qiang, Mingming;Tang, Bo
Alterations of cellular redox status are closely associated with physiological and pathological processes. Glutathione (GSH) and H2O2 should be the most representative redox couple in living cells. However, up to now, there is no way to reversibly detect GSH/H2O2. In this report, a near-infrared (NIR) fluorescent probe (Cy-O-Eb) for monitoring the changes of GSH/H2O2 levels in vivo was developed based on switching on-off a five-membered ring involved in ebselen. This probe could reversibly respond to GSH and H2O2 with high selectivity, sensitivity and mitochondrial targeting. It was successfully used to monitor the changes of redox status during apoptosis and the H2O2 changes at the wound margin in zebrafish larvae. Thus, the probe would provide an ideal tool for monitoring redox status changes and studying molecular events involved in redox regulation.
