Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation
Effect of regulated overexpression of the MADS domain factor AGL15 on flower senescence and fruit maturation
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DOI：
10.1104/pp.004721
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发表时间：
2002-09-01
期刊：
影响因子：
7.4
通讯作者：
Fernandez, DE
中科院分区：
文献类型：
--
作者：
Fang, SC;Fernandez, DE
我们研究了AGL15的受控过表达对生殖组织的影响，AGL15是MADS结构域调控因子家族的成员。利用分子和生理标记，我们证明了AGL15在拟南芥中的结构性过表达导致花被器官和果实发育过程中衰老程序的延迟和下调，并改变了种子的干燥过程。通过遗传杂交，我们表明成熟种子的失水率是由母体组织的遗传组成和生理状态决定的，而不是由胚胎决定的。为了确定AGL15水平升高对衰老程序影响最大的发育时间和/或地点，我们在不同启动子的控制下表达了AGL15。在衰老过程中或在离区细胞中的表达不会延迟花器官的衰老或脱落。利用糖皮质激素诱导的表达系统，我们证明了在花开放前后增加AGL15水平对于延缓衰老和延长花器官寿命是必要的。
We have examined the effect of regulated overexpression of AGL15, a member of the MADS domain family of regulatory factors, on reproductive tissues. Using molecular and physiological markers, we show that constitutive overexpression of AGL15 in Arabidopsis leads to delay and down-regulation of senescence programs in perianth organs and developing fruits and alters the process of seed desiccation. Through genetic crosses, we show that the rate of water loss in the maturing seeds is dictated by the genetic composition and physiological state of the maternal tissue, rather than the embryo. To define the developmental time and/or place when senescence programs are most affected by elevated AGL15 levels, we expressed AGL15 under the control of various promoters. Expression during senescence or in abscission zone cells did not produce delays in floral organ senescence or abscission. Using a glucocorticoid-inducible expression system, we show that an increase in AGL15 levels around the time of flower opening is necessary to delay senescence and increase floral organ longevity.