Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine.
Application of novel solid phase extraction-NMR protocols for metabolic profiling of human urine.
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DOI：
10.1039/c8fd00220g
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发表时间：
2019-08
影响因子：
3.4
通讯作者：
D. McGill;E. Chekmeneva;J. Lindon;Z. Takáts;J. Nicholson
中科院分区：
文献类型：
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作者：
D. McGill;E. Chekmeneva;J. Lindon;Z. Takáts;J. Nicholson
代谢物鉴定和注释程序对于发现指示表型或疾病状态的生物标志物是必要的，但这些过程可能会因含有数千种不同化合物的生物流体的绝对复杂性而受到瓶颈。在这里，我们描述了低成本的新型 SPE-NMR 方案，在天然和人造尿液混合物上使用不同的试剂盒和条件，产生独特的保留曲线，可用于代谢分析。我们发现，应用于尿液等生物流体的不同 SPE 方法可用于根据化合物分类或其他关键功能组选择性保留代谢物，通过未知物的浓缩和分馏减少峰重叠，从而有望更好地控制代谢物注释/识别过程。
Metabolite identification and annotation procedures are necessary for the discovery of biomarkers indicative of phenotypes or disease states, but these processes can be bottlenecked by the sheer complexity of biofluids containing thousands of different compounds. Here we describe low-cost novel SPE-NMR protocols utilising different cartridges and conditions, on both natural and artificial urine mixtures, which produce unique retention profiles useful for metabolic profiling. We find that different SPE methods applied to biofluids such as urine can be used to selectively retain metabolites based on compound taxonomy or other key functional groups, reducing peak overlap through concentration and fractionation of unknowns and hence promising greater control over the metabolite annotation/identification process.