Rat and mouse differences in gender-predominant expression of organic anion transporter (OAT1-3; SLC22A6-8) mRNA levels
Rat and mouse differences in gender-predominant expression of organic anion transporter (OAT1-3; SLC22A6-8) mRNA levels
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DOI：
10.1124/dmd.32.6.620
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发表时间：
2004-06-01
影响因子：
3.9
通讯作者：
Klaassen, CD
中科院分区：
文献类型：
--
作者：
Buist, SCN;Klaassen, CD
有机阴离子转运蛋白（燕麦）介导主动肾排泄的初始步骤，特别是近端小管细胞的底物摄取。尽管对大鼠燕麦进行了广泛的表征，但小鼠燕麦的表达模式实际上是未知的。本研究旨在确定小鼠Oat 1（Slc 22 a6），Oat 2（Slc 22 a7）和Oat 3（Slc 22 a8）mRNA的基础表达模式，将这些模式与大鼠中的模式进行比较，并表征小鼠Oat mRNA的出生后发育。从成年雄性和雌性129 J和C57 BL/6小鼠以及0至40日龄的雄性和雌性C57 BL/6小鼠中收集组织。燕麦mRNA水平通过分支DNA信号放大来确定。小鼠Oat 1 mRNA主要表达于两品系小鼠的肾脏，雄性小鼠占优势。小鼠Oat 2 mRNA水平在两个品系的肾脏中最高，没有性别优势。在这两种菌株中，Oat 3 mRNA在肾脏中最高，肝脏表达以男性为主。然而，只有129 J小鼠的雌性肾脏中的Oat 3 mRNA水平高于雄性肾脏。在出生后的发育过程中，Oat 1和Oat 2 mRNA水平在25日龄后开始上升。Oat 3 mRNA水平从出生到40日龄逐渐上升。Oat 2 mRNA在前40天增加了30倍，而Oat 1和Oat 3增加了约2倍。燕麦mRNA表达的最显着的物种差异是缺乏燕麦2女性占主导地位的小鼠肾脏和一个不太显着的燕麦3男性占主导地位的小鼠肝脏。除了一个显着的种属差异Oat 2的表达，许多相似之处被发现大鼠和小鼠的燕麦mRNA水平之间。
Organic anion transporters (Oats) mediate the initial step of active renal excretion, specifically substrate uptake into proximal tubule cells. Despite extensive characterization of rat Oats, mouse Oat expression patterns are virtually unknown. This study was designed to identify basal expression patterns of mouse Oat1 (Slc22a6), Oat2 (Slc22a7), and Oat3 (Slc22a8) mRNA, compare these patterns with those in rat, and characterize postnatal development of mouse Oat mRNA. Tissues were collected from adult male and female 129J and C57BL/6 mice, and male and female C57BL/6 mice 0 to 40 days of age. Oat mRNA levels were determined by branched DNA signal amplification. Mouse Oat1 mRNA was primarily expressed in kidney of both strains, with male predominance. Mouse Oat2 mRNA levels were highest in kidney of both strains without gender predominance. In both strains, Oat3 mRNA was highest in kidney, and liver expression was male-predominant. However, only 129J mice had higher Oat3 mRNA levels in female kidney than in male kidney. During postnatal development, both Oat1 and Oat2 mRNA levels began to rise after 25 days of age. Oat3 mRNA levels rose gradually from birth through 40 days of age. Oat2 mRNA increased 30-fold during the first 40 days, whereas Oat1 and Oat3 increased about 2-fold. The most notable species differences in Oat mRNA expression were a lack of Oat2 female predominance in mouse kidney and a less dramatic Oat3 male predominance in mouse liver. With the exception of a significant species difference in Oat2 expression, many similarities were found between rat and mouse Oat mRNA levels.