The influence of the tourniquet time on hernatological testing for antidoping purposes
The influence of the tourniquet time on hernatological testing for antidoping purposes
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DOI：
10.1055/s-2005-865749
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发表时间：
2006-05-01
影响因子：
2.5
通讯作者：
Guidi, G. C.
中科院分区：
文献类型：
--
作者：
Lippi, G.;Salvagno, G. L.;Guidi, G. C.
在精英和专业运动中，通过血液操作来优化有氧运动是一个严重的问题，识别血液兴奋剂的方法至今仍具有挑战性。在大多数情况下，目前的策略考虑进行第一阶段的分析，基于对血红蛋白或红细胞压积应用任意阈值，然后进行第二代血液测试，或采用个人血液学护照。为了确定分析前变量对运动员血液学特征的影响，我们比较了27名男性职业自行车运动员在平均握住止血带2.30+/-0.12次后的血红蛋白、红细胞压积和网织红细胞计数。在红细胞压积(+2.4%；p<；0.001)和血红蛋白测量(+1.4%；p<；0.001)方面观察到有统计学意义的差异，但在网织红细胞计数(-1.9%；p=0.170)方面没有显著差异。在27例中有4例(15%)，红细胞压积测量的变异性超过了4.1%的总误差的理想分析质量标准。目前的调查结果进一步突出了这样一种风险，即不执行严格和标准化的血样采集程序可能会增加假阳性检测的数量，并可能导致对少数血细胞比容或血红蛋白值自然升高的干净运动员进行不适当的处罚。由于分析前阶段的生物变异性很小，对变化的敏感性较小，在实验室检测中，血红蛋白浓度可能比红细胞压积更适合作为检测血液兴奋剂的参数。
Hematological manipulation to optimize aerobic performances is a serious problem in elite and professional sports and the approach to identify blood doping is as yet challenging. In most cases, the current strategy contemplates a first stage of analysis, based on the application of arbitrary threshold for hemoglobin or hematocrit, followed by second-generation blood tests, or the adoption of an individual hematological passport. To establish the influence of preanalytical variables on the athletes' hematological profile, we compared hemoglobin, hematocrit, and reticulocytes count in 27 male professional cyclists after a mean time of 2.30 +/- 0.12 tourniquet holding. Statistically significant differences were observed for hematocrit (+ 2.4%; p < 0.001) and hemoglobin measurements (+ 1.4%; p < 0.001), but not for the reticulocytes count (- 1.9%; p = 0.170). In 4 out of 27 cases (15%), the variability of the hematocrit measurement exceeded the 4.1% desirable analytical quality specification for total error. Results of the present investigation further highlight the risk that unfulfillment of rigorous and standardized procedures for collection of blood specimens might increase the number of false positive testing and might lead to inappropriate sanctioning of a minority of clean athletes with hematocrit or hemoglobin values naturally elevated. Owing to the minor biological variability and the lesser susceptibility to variation of the preanalytical phase, the hemoglobin concentration might be a more suitable parameter than hematocrit for inclusion within laboratory testing to identify blood doping.