Two-photon standing-wave fluorescence correlation spectroscopy
Two-photon standing-wave fluorescence correlation spectroscopy
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DOI：
10.1364/ol.32.002121
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发表时间：
2007-08-01
期刊：
影响因子：
3.6
通讯作者：
Bardeen, Christopher J.
中科院分区：
文献类型：
--
作者：
Hanson, Kerry M.;Davis, Sara K.;Bardeen, Christopher J.
介绍了一种结合双光子激发和驻波干涉的荧光相关光谱实验。实验相关函数可以使用一个简单的表达式进行分析，该表达式涉及（1）具有时间常数τ（f）的指数衰减，其反映了干涉条纹上的扩散，以及（2）具有时间常数τ（ω）的较长寿命衰减，其反映了焦斑内外的扩散。用此方法测定了罗丹明110在水和乙二醇中的扩散。在两个不同的时间和长度尺度上同时测量扩散的能力使得该实验在怀疑异常扩散的环境中特别有用。(C)2007年，美国光学学会。
A fluorescence correlation spectroscopy experiment that combines two-photon excitation and a standing-wave interference pattern is presented. The experimental correlation function can be analyzed using a simple expression involving (1) an exponential decay with time constant tau(f), which reflects diffusion across the interference fringes, and (2) a longer-lived decay with time constant tau(omega), which reflects diffusion in and out of the focal spot. The diffusion of Rhodamine 110 in water and ethylene glycol is measured using this method. The ability to simultaneously measure diffusion on two different time and lengthscales makes this experiment especially useful in environments where anomalous diffusion is suspected. (C) 2007 Optical Society of America.