Effect of ectomycorrhizal fungal species on population growth and food preference of a fungivorous nematode
Effect of ectomycorrhizal fungal species on population growth and food preference of a fungivorous nematode
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DOI：
10.1007/s00572-021-01063-0
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发表时间：
2022-01
期刊：
影响因子：
3.9
通讯作者：
Y. Kitagami;Y. Matsuda
中科院分区：
文献类型：
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作者：
Y. Kitagami;Y. Matsuda
食真菌线虫可以利用森林土壤中的外生菌根（ECM）真菌作为食物资源，并且它们可能在森林生态系统中建立密切的捕食者与被捕食者关系。然而，ECM真菌物种对食真菌线虫生长的影响的研究很少。为了确定食真菌线虫的繁殖和对 ECM 真菌的偏好，我们研究了食真菌线虫滑刃线虫的体外种群生长和食物吸引力。在含有四种 ECM 真菌的培养基上：Cenochocum geophilum、Pisolithustinctorius、Rhizopogonroseolus 和 Suillus capsulatus。单个线虫以在改良的 Melin-Norkrans 琼脂培养基上生长的所有四种 ECM 真菌的菌丝为食。 P 上的线虫数量明显较低。 Tinctorius 比所有其他真菌物种更有效。其他三个物种产生了类似的种群增长率，其中 S.颗粒细胞在第 2、3 和 4 周时产生最多数量的线虫，C. geophilumandR。 8 周后，roseolus 产生的数量最多。在线虫长度类别的直方图中，对 P 拟合了单峰模式。廷克托瑞斯和 R. Roseolus，但 C 适合双峰模式。地球亲和者S.颗粒细胞通过西尔弗曼测试。线虫对S的吸引力。颗粒菌显着高于其他 ECM 真菌。我们的研究结果表明，线虫的繁殖和体型大小取决于 ECM 真菌种类。食真菌线虫和 ECM 真菌之间的捕食-被捕食关系可能会加速森林生态系统的养分循环。
Fungivorous nematodes can use ectomycorrhizal (ECM) fungi as food resources in forest soils, and they may establish close predator–prey relationships in forest ecosystems. However, the effect of ECM fungal species on the growth of fungivorous nematodes is poorly studied. To identify fungivorous nematode propagation and preference for ECM fungi, we investigated the in vitro population growth and food attraction of the fungivorous nematodeAphelenchoidessp. on media with four ECM fungal species:Cenococcum geophilum,Pisolithus tinctorius,Rhizopogon roseolusandSuillus granulatus. Individual nematodes were fed on hyphae of all four ECM fungal species grown on modified Melin-Norkrans agar media. Nematode numbers were significantly lower onP. tinctoriusthan on all other fungal species. The other three species produced similar population growth rates, withS. granulatusproducing the greatest number of nematodes at 2, 3 and 4 weeks andC. geophilumandR. roseolusproducing the largest number after 8 weeks. In the histogram for nematode length classes, a unimodal pattern was fitted forP. tinctoriusandR. roseolus, but a bimodal pattern was fitted forC. geophilumandS. granulatusby the Silverman test. The attraction of nematodes toS. granulatuswas significantly higher than that to other ECM fungi. Our findings suggest that the propagation and body size of nematodes are ECM fungal species dependent. Predator–prey relationships between fungivorous nematodes and ECM fungi may accelerate nutrient cycles in forest ecosystems.