Localization of myosin in the preimplantation mouse embryo.
Localization of myosin in the preimplantation mouse embryo.
复制标题
植入前小鼠胚胎中肌球蛋白的定位。
DOI：
10.1016/0012-1606(83)90021-0
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发表时间：
1983
影响因子：
2.7
通讯作者：
J. Sobel
中科院分区：
文献类型：
--
作者：
J. Sobel
用间接免疫荧光技术对小鼠植入前胚胎的细胞骨架进行了定位。2-和4-细胞胚胎的卵裂球表现出连续带皮质肌球蛋白在其顶端的边界，而没有皮质肌球蛋白是检测到的细胞并置区域。在8-细胞，16-细胞，和先进的桑椹胚阶段的胚胎外组的细胞也具有连续的皮质肌球蛋白的顶端带，而内组的并列细胞通常没有检测到皮质肌球蛋白。这些意见的可能意义进行了讨论。
Myosin was localized in cytoskeletal preparations of preimplantation mouse embryos using indirect immunofluorescent techniques. Blastomeres of 2- and 4-cell embryos exhibited a continuous band of cortical myosin in their apical borders while no cortical myosin was detectable in regions of cell apposition. In 8-cell, 16-cell, and advanced morula-stage embryos the outer group of cells was also characterized by a continuous apical band of cortical myosin, whereas the inner group of apposed cells usually showed no detectable cortical myosin. The possible significance of these observations is discussed.