Brassinosteroids alleviate nanoplastic toxicity in edible plants by activating antioxidant defense systems and suppressing nanoplastic uptake
Brassinosteroids alleviate nanoplastic toxicity in edible plants by activating antioxidant defense systems and suppressing nanoplastic uptake
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DOI：
10.1016/j.envint.2023.107901
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发表时间：
2023-03-30
影响因子：
11.8
通讯作者：
Wang, Tian
中科院分区：
文献类型：
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作者：
Gao, Mingyang;Wang, Zhongtang;Wang, Tian
作为全球关注的新兴污染物，吸收的纳米塑料可能会对植物发育和养分吸收产生负面影响，从而降低产量。如果纳米塑料转移到植物的可食用部分，大量摄入时可能会对人类健康构成威胁。虽然纳米塑料引起的植物毒性越来越受到关注，但如何抑制纳米塑料在植物中的积累并减少随后的不利影响却知之甚少。在这里，我们研究了不同植物物种中聚苯乙烯纳米塑料（PS-NP）的吸收和积累以及油菜素类固醇在减轻PS-NP毒性中的作用。油菜素类固醇抑制番茄果实中 PS-NP 的积累，逆转 PS-NP 诱导的植物毒性，促进植物生长，增加鲜重和株高。油菜素类固醇还逆转了PS-NP（包括TIP2-1、TIP2-2、PIP2-6、PIP2-8、PIP2-9、SIP2-1和NIP1-2）对水通道蛋白相关基因的诱导，提供了PS-NP在可食用部分和抑制目标中积累的潜在应激机制。在转录组学分析中，油菜素类固醇增强了脂肪酸和氨基酸的代谢和合成。总之，外源施用50 nM油菜素内酯减轻了PS-NPs对植物的不利影响，外源施用油菜素内酯可能是减少PS-NP引起的植物毒性的有效手段。
As emerging pollutants of global concern, absorbed nanoplastics might have negative impacts on plant development and nutrient uptake, thereby decreasing yields. If nanoplastics are transferred to the edible parts of plants, they may pose a threat to human health when large quantities are ingested. While nanoplastic-induced phytotoxicity is attracting increasing attention, little is known about how to inhibit nanoplastic accumulation in plants and reduce the subsequent adverse effects. Here we investigated the absorption and accumulation of polystyrene nanoplastics (PS-NPs) in different plant species and the role of brassinosteroids in alleviating PS-NP toxicity. Brassinosteroids inhibited accumulation of PS-NPs in tomato fruit and reversed PS-NP-induced phytotoxicity to promote plant growth and increase fresh weight and plant height. Brassinosteroids also reversed the induction of aquaporin-related genes by PS-NPs including TIP2-1, TIP2-2, PIP2-6, PIP2-8, PIP2-9, SIP2-1, and NIP1-2, providing a potential stress mechanism by which PS-NPs accumulate in the edible parts and targets for inhibition. In transcriptomic analyses, brassinosteroids enhanced fatty acid and amino acid metabolism and synthesis. In conclusion, exogenous application of 50 nM brassinosteroids alleviated the adverse effects of PS-NPs on plants, and exogenous application of brassinosteroids might be an effective means to minimize PS-NP-induced phytotoxicity.