Transforming growth factor-beta. Murine glomerular receptors and responses of isolated glomerular cells.
Transforming growth factor-beta. Murine glomerular receptors and responses of isolated glomerular cells.
复制标题
转化生长因子-β。
DOI：
10.1172/jci113996
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发表时间：
1989
期刊：
影响因子：
--
通讯作者：
Striker,GE
中科院分区：
文献类型：
--
作者：
MacKay,K;Striker,LJ;Stauffer,JW;Doi,T;Agodoa,LY;Striker,GE
常驻肾小球细胞的增殖和系膜基质的积累是在许多进行性肾小球疾病的过程中观察到的组织学异常。我们探讨了转化生长因子-β (TGF-β) 对这些过程的潜在调节作用。我们发现培养的小鼠肾小球内皮细胞、系膜细胞和上皮细胞以及分离的完整大鼠肾小球具有 TGF-β 的高亲和力受体。我们还发现，尽管 TGF-β 持续抑制肾小球内皮和上皮细胞的增殖，但它充当系膜细胞增殖的双功能调节剂。 TGF-β显着增加肾小球系膜细胞胶原蛋白和纤连蛋白的产生，而肾小球上皮细胞中仅纤连蛋白的产生增加。完整肾小球和每种肾小球细胞类型上存在 TGF-β 受体，以及这些细胞对 TGF-β 的反应性，这表明体内驻留肾小球细胞和 TGF-β 之间可能发生潜在的重要相互作用。
Proliferation of resident glomerular cells and the accumulation of mesangial matrix are histologic abnormalities which are observed in the course of many progressive glomerular diseases. We explored the potential regulatory effects of transforming growth factor-beta (TGF-beta) on these processes. We found that cultured mouse glomerular endothelial, mesangial, and epithelial cells as well as isolated intact rat glomeruli possess high-affinity receptors for TGF-beta. We also found that, although TGF-beta consistently inhibited the proliferation of glomerular endothelial and epithelial cells, it acted as a bifunctional regulator of mesangial cell proliferation. TGF-beta significantly increased the production of collagen and fibronectin by glomerular mesangial cells whereas only fibronectin production was augmented in glomerular epithelial cells. The presence of TGF-beta receptors on intact glomeruli and on each glomerular cell type and the demonstrated responsiveness of these cells to TGF-beta combine to suggest that potentially important interactions may occur between resident glomerular cells and TGF-beta in vivo.Images