CATECHOLAMINE-SYNTHESIZING ENZYMES AND NEUROPEPTIDES IN RAT-HEART EPICARDIAL GANGLIA - AN IMMUNOHISTOCHEMICAL STUDY
CATECHOLAMINE-SYNTHESIZING ENZYMES AND NEUROPEPTIDES IN RAT-HEART EPICARDIAL GANGLIA - AN IMMUNOHISTOCHEMICAL STUDY
复制标题
DOI：
10.1007/bf01047451
复制
发表时间：
1990-12-01
期刊：
影响因子：
--
通讯作者：
MORAVEC, J
中科院分区：
文献类型：
--
作者：
FORSGREN, S;MORAVEC, M;MORAVEC, J
使用免疫组织化学技术和抗儿茶酚胺合成酶酪氨酸羟化酶（TH）和多巴胺-β-受体的抗体检查大鼠心脏的心外膜下心房神经节。羟化酶（DBH）、神经肽P物质（SP）、降钙素基因相关肽（CGRP）、神经肽Y（NPY）、血管活性肠肽（VIP）和甲硫氨酸-5-脑啡肽（ENK）。部分神经节细胞呈DBH样免疫反应性（LI）和NPY-LI，而这些细胞不呈TH-、VIP-、CGRP-、SP-或ENK-LI。在神经节中观察到表现出强烈TH-LI的小细胞群，对应于文献中称为含儿茶酚胺细胞的细胞，有时是小的强荧光细胞。这些细胞的一个亚群表现出免疫反应的神经肽之一，即SP。只有少数的细胞显示TH-LI显示DBH-LI。神经节内存在SP-、CGRP-、DBH-和TH-阳性的神经纤维，其中有些纤维与神经节细胞或TH-阳性细胞紧密相连。观察提供了新的信息，儿茶酚胺合成酶/神经肽的神经节和含儿茶酚胺的细胞和相关的神经纤维的大鼠心脏心外膜神经节的表达。
The subepicardial atrial ganglia of rat hearts were examined using immunohistochemical techniques and antibodies against the catecholamine-synthetic enzymes tyrosine hydroxylase (TH) and dopamine-.beta.-hydroxylase (DBH), and the neuropeptides substance P (SP), calcitonin gene-related peptide (CGRP), neuropeptide Y (NPY), vasoactive intestinal polypeptide (VIP) and met-5-enkephalin (ENK). Some of the ganglion cells present in the ganglia exhibited DBH-like immunoreactivity (LI) and NPY-LI, whilst these cells never exhibited TH-, VIP-, CGRP-, SP- or ENK-LI. Groups of small cells exhibiting an intense TH-LI, corresponding to cells referred to as catecholamine-containing cells and sometimes small intensely fluorescent cells in the literature, were observed in the ganglia. A subpopulation of these cells exhibited immunoreactivity to one of the neuropeptides tested, namely SP. Only a few of the cells showing TH-LI displayed DBH-LI. Nerve fibres showing SP-, CGRP-, DBH- and TH-LI were present in the ganglia; some of these fibres being closely associated with the ganglion cells or with the cells showing TH-LI. The observations provide new information on the catecholamine-synthetic enzyme/neuropeptide expression of the ganglion and catecholamine-containing cells and of the associated nerve fibres of rat heart subepicardial ganglia.