Lipolytic activity of Campylobacter pylori: effect of sofalcone.
Lipolytic activity of Campylobacter pylori: effect of sofalcone.
复制标题
幽门弯曲杆菌的脂肪分解活性：索法酮的作用。
作者：
Slomiany,BL;Nishikawa,H;Piotrowski,J;Okazaki,K;Slomiany,A
研究了能降解胃粘膜脂质的幽门弯曲菌的脂解活性。从接受内窥镜检查的患者的胃窦粘膜活检标本中培养的细菌菌落用盐水洗涤并通过灭菌过滤器;使用甘油三油酸酯和二棕榈酰磷脂酰胆碱作为底物，检查滤液的脂肪酶和磷脂酶A活性。结果表明，这两种酶都存在于C.幽门滤液酶在37 °C和7.0-7.4的pH范围内表现出最佳活性。甘油三酯降解的主要产物是甘油单油酸酯，而溶血磷脂酰胆碱是由磷脂酰胆碱降解产生的。幽门螺杆菌滤液的脂肪酶活性可被抗溃疡药物索法酮抑制，索法酮浓度为200 µg/ml时可使甘油三酯水解减少43%。然而，索法酮对幽门螺杆菌磷脂酶A的抑制活性即使有，也仅为轻微的。提示幽门螺杆菌感染可损害胃粘膜脂质的完整性。
The lipolytic activity ofCampylobacter pyloricapable of gastric mucosal lipid degradation was investigated. The colonies of bacteria, cultured from antral mucosal biopsy specimens of patients undergoing endoscopy, were washed with saline and passed through a sterilization filter; the filtrate was examined for lipase and phospholipase A activities, using glycerol trioleate and dipalmitoyl phosphatidylcholine as substrates. The obtained results revealed that both enzymes are present in theC. pylorifiltrate. The enzymes exhibited optimal activity at 37 °C and the pH range of 7.0–7.4. The major product of the triglyceride degradation was glycerol monoleate, while lysophosphatidylcholine resulted from the degradation of phosphatidylcholine. The lipase activity of theC pylorifiltrate was inhibited by an antiulcer drug, sofalcone, which at a concentration of 200 µg/ml caused a 43 % reduction in the triglyceride hydrolysis. The sofalcone, however, showed only marginal, if any, inhibitory activity towardC pyloriphospholipase A. The results suggest thatC pyloriinfection may be detrimental to the integrity of gastric mucosal lipids.