The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion molecule promotes interaction with nerves
The spermatogenic Ig superfamily/synaptic cell adhesion molecule mast-cell adhesion molecule promotes interaction with nerves
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DOI：
10.4049/jimmunol.174.11.6934
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发表时间：
2005-06-01
影响因子：
4.4
通讯作者：
Kitamura, Y
中科院分区：
文献类型：
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作者：
Furuno, T;Ito, A;Kitamura, Y
神经 - 肥大细胞相互作用参与体内平衡调节和病理调节。维持这种关联的分子尚未得到确认。由于突触细胞黏附分子（SynCAM，又称生精免疫球蛋白超家族（SgIGSF））在神经和肥大细胞上均有表达，且能进行同源性结合，因此该分子可能是一个候选对象。为验证这一可能性，将表达或不表达SgIGSF/SynCAM的肥大细胞与表达SgIGSF/SynCAM的颈上神经节神经元共同培养，并对附着在神经突上的肥大细胞数量进行计数。针对SgIGSF/SynCAM的阻断性抗体可剂量依赖性地抑制表达SgIGSF/SynCAM的肥大细胞（即来自野生型小鼠的骨髓源性肥大细胞（BMMC））的附着。不表达SgIGSF/SynCAM的肥大细胞，即来自小眼畸形转录因子缺陷型小鼠的BMMC以及BMMC衍生的细胞系IC - 2细胞，在附着到神经突方面存在缺陷，而转染SgIGSF/SynCAM可使其恢复正常。当神经被蝎毒特异性激活时，附着的IC - 2细胞中有四分之一在数十秒后出现钙离子动员，而异位表达SgIGSF/SynCAM可使这一比例翻倍。在神经突与野生型BMMC的接触点处，SgIGSF/SynCAM在神经突和BMMC中均局部聚集。肥大细胞上的SgIGSF/SynCAM似乎主要介导附着并促进与神经的通讯。
Nerve-mast cell interaction is involved in both homeostatic and pathologic regulations. The molecules that sustain this association have not been identified. Because synaptic cell adhesion molecule (SynCAM), alternatively named spermatogenic Ig superfamily (SgIGSF), is expressed on both nerves and mast cells and because it binds homophilically, this molecule may be a candidate. To examine this possibility, mast cells with or without SgIGSF/SynCAM were cocultured with superior cervical ganglion neurons that express SgIGSF/SynCAM, and the number of mast cells attached to neurites was counted. The attachment of mast cells with SgIGSF/SynCAM, i.e., bone marrow-derived mast cells (BMMC) from wild-type mice, was inhibited dose-dependently by blocking Ab to SgIGSF/SynCAM. Mast cells without SgIGSF/SynCAM, i.e., BMMC from microphthalmia transcription factor-deficient mice and BMMC-derived cell line IC-2 cells, were defective in attachment to neurite, and transfection with SgIGSF/SynCAM normalized this. When the nerves were specifically activated by scorpion venom, one-quarter of the attached IC-2 cells mobilized Ca2+ after a few dozen seconds, and ectopic SgIGSF/SynCAM doubled this proportion. At points of contact between neurites and wild-type BMMC, SgIGSF/SynCAM was locally concentrated in both neurites and BMMC. SgIGSF/SynCAM on mast cells appeared to predominantly mediate attachment and promote communication with nerves.