Renal potassium channels: Function, regulation, and structure
Renal potassium channels: Function, regulation, and structure
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DOI：
10.1046/j.1523-1755.2001.060002436.x
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发表时间：
2001-08-01
影响因子：
19.6
通讯作者：
Giebisch, G
中科院分区：
文献类型：
--
作者：
Giebisch, G
肾小管中的许多转运功能依赖于钾（K）通道。不仅钾分泌和维持外部钾平衡依赖于主小管细胞中的钾通道活性，而且钾通道还调节细胞体积，它们是所有小管细胞中细胞功能的组成部分，因为它们在影响许多带电溶质跨膜运动的细胞负电位的产生中起关键作用。此外，跨厚升支（TAL）的顶膜的K回收在此小管段的NaCl重吸收的控制中起着重要的作用。通过结合几种策略，我们对肾脏K通道的结构和功能的理解取得了重大进展。这些研究包括在单个肾小管中的转运研究，应用膜片钳技术探索天然肾小管中单个K通道的特性，以及克隆和表达多种肾脏来源的K通道。从这些调查的见解承诺提供一个更深入的了解K通道参与许多不同的小管功能的机制。
Many transport functions in renal tubules depend on potassium (K) channels. Not only does K secretion and the maintenance of external K balance depend on K channel activity in principal tubule cells, but K channels also regulate cell volume, they are an integral party of cell function in all tubule cells because of their key role in the generation of the cell-negative electrical7 potential that affects the transmembrane movement of many charged solutes. Moreover, the recycling of K across the apical membrane of the thick ascending limb (TAL) plays an important role in the control of NaCl reabsorption in this tubule segment. Significant progress in our understanding of the structure and function of renal K channels has become possible by combining several strategies. These include transport studies in single tubules, application of the patch-clamp technique for exploring the properties of single K channels in native tubules and the cloning, and expression of diverse K channels of renal origin. Insights from these investigations promise to provide a deeper understanding of the mechanism by which K channels participate in many diverse tubule functions.