Activation of Orexin B receptors in the pontine Kolliker-Fuse nucleus modulates pre-inspiratory hypoglossal motor activity in rat
Activation of Orexin B receptors in the pontine Kolliker-Fuse nucleus modulates pre-inspiratory hypoglossal motor activity in rat
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DOI：
10.1016/j.resp.2007.06.004
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发表时间：
2007-11-15
影响因子：
2.3
通讯作者：
Gestreau, Christian
中科院分区：
文献类型：
--
作者：
Dutschmann, Mathias;Kron, Miriam;Gestreau, Christian
食欲素（剪接变体A和B）是下丘脑神经肽，在控制唤醒和营养方面具有重要功能。食欲素缺乏与嗜睡症和睡眠呼吸障碍密切相关。然而，食欲素，特别是食欲素-B（OXB），在呼吸中心控制上气道通畅的作用是不太确定的。在本研究中，我们进行了微量注射OXB到脑桥背外侧的Kolliker-Ehrenus核（KF），因为这个核是特别涉及上气道肌肉的运动前控制。在原位灌注脑干制备中分析了OXB介导的对心脏、膈神经（PNA）和舌下神经（XII-A）活动的影响。将OXB注入KF引起呼吸频率显著增加。重要的是，OXB引起特别延长的第十二神经的吸气前放电，而KF微量注射后没有观察到心血管反应。总之，KF中的OXB对XII前运动神经元发挥兴奋作用。由于吸气前活动的XII是重要的，在吸气过程中上气道阻力的减少，我们得出结论，在KF中的OXB释放有很强的影响，在生理和病理生理条件下的上气道通畅的状态依赖性控制。(C)2007 Elsevier B. V.保留所有权利。
Orexins (splice variants A and B) are hypothalamic neuropeptides that have essential functions in control of arousal and nutrition. Lack of Orexins is strongly associated with narcolepsy and sleep disordered breathing. However, the role of Orexins and particularly that of Orexin-B (OXB), in respiratory centres controlling upper-airway patency are less defined. In the present study we performed microinjections of OXB into the pontine Kolliker-Fuse nucleus (KF) of the dorsolateral pons, since this nucleus is particularly involved in the pre-motor control of upper airway muscles. The OXB mediated effects on heart, phrenic (PNA) and hypoglossal (XII-A) nerve activities were analysed in an in situ perfused brainstem preparation. Injection Of OXB into the KF evoked significant augmentation of the respiratory frequency. Importantly, OXB provoked particularly prolonged pre-inspiratory discharge of the XII nerve, while no cardiovascular response was observed after KF microinjections. In summary, OXB in the KF exerts an excitatory effect on XII pre-motoneurones. Since pre-inspiratory activity of the XII is important for the decrease in upper airway resistance during inspiration, we conclude that OXB release in the KF has strong implications in the state-dependent control of upper airway patency under physiological and pathophysiological conditions. (C) 2007 Elsevier B.V. All rights reserved.