Production and characterization of a monoclonal antibody that binds Reed-Sternberg cells.
Production and characterization of a monoclonal antibody that binds Reed-Sternberg cells.
复制标题
结合里德-斯滕伯格细胞的单克隆抗体的生产和表征。
作者：
T. Hecht;D. Longo;J. Cossman;J. Bolen;S. Hsu;M. Israel;R. Fisher
用L428霍奇金病组织培养细胞株免疫小鼠，产生一种小鼠单抗，命名为HEFI-1。18例霍奇金病中有18例仅选择性地染色Reed-Sternberg或Hodgkin细胞，包括结节硬化型、混合细胞型和淋巴细胞耗竭亚型。HEFI-1在正常肺、脑、唾液腺、甲状腺、胆、胰腺、肝、睾丸、乳腺、子宫内膜或肾脏中未见任何细胞染色。在正常扁桃体、结肠和增生性胸腺中，淋巴滤泡边缘可见罕见的大细胞。14例B细胞性非霍奇金淋巴瘤均未见细胞表达，而11例非霍奇金淋巴瘤中有3例表达T细胞标志的恶性细胞也被HEFI-1染色。组织培养细胞系包括T细胞急性淋巴细胞白血病细胞系MOLT4和CEM、组织细胞系U-937和羊膜细胞系Wish不染色。7株EB病毒(EBV)阳性的淋巴母细胞系被HEFI-1染色，而3株EBV阳性的非洲Burkitt淋巴瘤细胞株和3株EBV-American Burkitt细胞株均未见染色。HEFI-1不会阻断L428细胞刺激混合淋巴细胞反应的能力，也不会作为丝裂原诱导的人类T细胞增殖反应的辅助细胞发挥作用。未观察到HEFI-1细胞表面抗原对L428细胞的调节作用。HEFI-1特异性免疫沉淀来自L428和EBV+淋巴母细胞系的约120,000道尔顿的细胞表面蛋白。HEFI-1单抗不仅可用于霍奇金氏病的诊断、分期和潜在的治疗，还可用于确定Reed-Sternberg细胞的来源。
A murine monoclonal antibody, termed HeFi-1, was produced after immunization with the L428 Hodgkin's disease tissue culture cell line. HeFi-1 selectively stained only the Reed-Sternberg or Hodgkin's cells in 18 of 18 cases of Hodgkin's disease, including the nodular sclerosis, mixed cellularity, and lymphocyte-depleted histologic subtypes. HeFi-1 did not stain any cells in normal lung, brain, salivary gland, thyroid, gall bladder, pancreas, liver, testis, breast, endometrium, or kidney. Rare large cells at the edge of the lymphoid follicles were stained in normal tonsil, colon, and hyperplastic thymus. There was no staining of any cells in 14 cases of B cell non-Hodgkin's lymphoma; however, the malignant cells in three of 11 cases of non-Hodgkin's lymphoma which appeared to express T cell markers were also stained with HeFi-1. Tissue culture cell lines including the T cell acute lymphocytic leukemia lines MOLT4 and CEM, the histiocytic cell line U-937, and the amniotic cell line WISH were not stained. Seven Epstein Barr virus (EBV)-positive lymphoblastoid cell lines were stained with HeFi-1, but there was no staining of three EBV+ African Burkitt's lymphoma cell lines or three EBV- American Burkitt's cell lines. HeFi-1 did not block the ability of the L428 cells to stimulate a mixed lymphocyte reaction or function as accessory cells for mitogen-induced human T cell proliferative responses. Modulation of the HeFi-1 cell surface antigen on the L428 cells was not observed. HeFi-1 specifically immunoprecipitated a cell surface protein of approximately 120,000 daltons from both the L428 and EBV+ lymphoblastoid cell lines. HeFi-1 monoclonal antibody should prove useful not only in the diagnosis, staging, and potential therapy of Hodgkin's disease, but also for determining the cell of origin of the Reed-Sternberg cell.