Characterization of zebrafish intestinal smooth muscle development using a novel sm22α-b promoter.
Characterization of zebrafish intestinal smooth muscle development using a novel sm22α-b promoter.
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DOI：
10.1002/dvdy.22420
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发表时间：
2010-11
期刊：
影响因子：
--
通讯作者：
Pack M
中科院分区：
文献类型：
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作者：
Seiler C;Abrams J;Pack M
平滑肌细胞为许多组织提供结构支持，并控制基本的生理过程，如血压和胃肠动力。关于肠平滑肌发育的早期阶段及其与调节肠运动的肠神经系统发育的关系，人们知之甚少。在这里，我们报告了一个进化上保守的523个碱基对的调控元件内的启动子的斑马鱼sm 22 α-B（transgelin 1）基因，指导转基因表达的平滑肌细胞的肠和其他组织。比较基因组分析确定了一个保守的基序在这个元素组成的两个血清反应因子结合位点，也存在于许多哺乳动物平滑肌基因的启动子。我们建立了一个稳定的表达GFP的平滑肌细胞系，并使用此线来描述不同肠平滑肌层内的细胞之间的谱系关系及其与肠神经系统（ENS）的共同发展。
Smooth muscle cells provide structural support for many tissues and control essential physiological processes, such as blood pressure and gastrointestinal motility. Relatively little is known about early stages of intestinal smooth muscle development and its relationship to the development of the enteric nervous system, which regulates intestinal motility. Here, we report an evolutionarily conserved 523 base pair regulatory element within the promoter of the zebrafish sm22α-b (transgelin1) gene that directs transgene expression in smooth muscle cells of the intestine and other tissues. Comparative genomic analysis identified a conserved motif within this element consisting of two Serum Response Factor binding sites that is also present in the promoters of many mammalian smooth muscle genes. We established a stable line expressing GFP in smooth muscle cell and used this line to describe lineage relationships among cells within different intestinal smooth muscle layers and their co-development with the enteric nervous system (ENS).