Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single-Molecule Atomic Force Microscopy.
Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single-Molecule Atomic Force Microscopy.
复制标题
使用单分子原子力显微镜表征活细胞中粘附蛋白的生物物理特性。
DOI：
10.1007/978-1-0716-2851-5_4
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Sivasankar,Sanjeevi
中科院分区：
文献类型：
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作者：
Priest,AndrewVae;Sivasankar,Sanjeevi
细胞粘附蛋白在组织的形成、再生和维持中起重要作用。然而，细胞调节粘附蛋白的构象和结合特性的分子机制知之甚少。这些生物物理性质可以解决，单分子分辨率，使用原子力显微镜（AFM）。在这里，我们概述了如何AFM力测量可用于研究的构象，细胞骨架连接，结合强度，和力依赖的键寿命的粘附蛋白在活细胞中。
Cell adhesion proteins play essential roles in the formation, regeneration, and maintenance of tissue. However, the molecular mechanisms by which cells regulate the conformation and binding properties of adhesion proteins are poorly understood. These biophysical properties can be resolved, with single-molecule resolution, using atomic force microscopy (AFM). Here, we outline how AFM force measurements can be used to study the conformation, cytoskeletal linkage, binding strength, and force-dependent bond lifetimes of adhesion proteins in live cells.