Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila.
Rootletin organizes the ciliary rootlet to achieve neuron sensory function in Drosophila.
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DOI：
10.1083/jcb.201502032
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发表时间：
2015-10-26
期刊：
影响因子：
--
通讯作者：
Megraw TL
中科院分区：
文献类型：
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作者：
Chen JV;Kao LR;Jana SC;Sivan-Loukianova E;Mendonça S;Cabrera OA;Singh P;Cabernard C;Eberl DF;Bettencourt-Dias M;Megraw TL
果蝇Rootaoke在感觉神经元中组织小根，在那里它传递多种感觉输入并维持基体凝聚力，但它不是纤毛稳定所必需的。纤毛对于细胞信号传导和感觉感知是必不可少的。在许多细胞类型中，称为纤毛根的细胞骨架结构将纤毛连接到细胞体。以前的研究表明，小根支持一些纤毛的长期稳定性。在这里，我们报告，果蝇Rootaoke（根），唯一的直系同源的哺乳动物旁系Rootaoke和C-Nap 1，组装成不同长度的感觉神经元亚型之间的小根。根突变神经元缺乏小根，并具有显著受损的感觉功能，导致与机械感觉和化学感觉相关的行为缺陷。根是基体的凝聚力所必需的，但纤毛结构在根突变神经元中表现正常。然而，我们发现，正常的小根组装需要中心粒。根的N端包含一个保守的结构域，是根在体内功能所必需的。异位表达的根驻留在精母细胞中的母亲中心粒的基础上，并不对称地定位于成神经细胞中的母亲中心体，两者都需要Bld 10，一种具有不同功能的基体蛋白。
Drosophila Rootletin organizes rootlets in sensory neurons, where it transmits multiple sensory inputs and maintains basal body cohesion, yet it is not required for cilium stability. Cilia are essential for cell signaling and sensory perception. In many cell types, a cytoskeletal structure called the ciliary rootlet links the cilium to the cell body. Previous studies indicated that rootlets support the long-term stability of some cilia. Here we report that Drosophila melanogaster Rootletin (Root), the sole orthologue of the mammalian paralogs Rootletin and C-Nap1, assembles into rootlets of diverse lengths among sensory neuron subtypes. Root mutant neurons lack rootlets and have dramatically impaired sensory function, resulting in behavior defects associated with mechanosensation and chemosensation. Root is required for cohesion of basal bodies, but the cilium structure appears normal in Root mutant neurons. We show, however, that normal rootlet assembly requires centrioles. The N terminus of Root contains a conserved domain and is essential for Root function in vivo. Ectopically expressed Root resides at the base of mother centrioles in spermatocytes and localizes asymmetrically to mother centrosomes in neuroblasts, both requiring Bld10, a basal body protein with varied functions.