Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
Mouse heavy chain variable regions: nucleotide sequence of a germ-line VH gene segment.
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小鼠重链可变区：种系VH基因片段的核苷酸序列。
DOI：
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发表时间：
1981
影响因子：
14.9
通讯作者：
François Rougeon
中科院分区：
文献类型：
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作者：
R. Ollo;Charles Auffray;Jean;François Rougeon
我们在载体 Charon 4A 中构建了 Balb/c 小鼠胚胎 DNA 文库。在文库中搜索与 UPC10 重链 mRNA 的克隆 cDNA 的 VH 区同源的序列。在本文中，我们描述了此类克隆之一（VH441）的结构和部分核苷酸序列。该种系基因的核苷酸序列表明它编码X44和J601半乳聚糖结合VH区的氨基酸1-98，但它与UPC10 VH片段有四个单碱基变化不同。 VH 基因似乎在与 UPC10 前体序列相同的前体序列内包含 101 个碱基长的间插序列。 V基因的3'非编码序列包含在胚胎V DNA片段中发现的两个保守序列，CACAGTG和ACATGAACC，其间隔23个核苷酸，以及序列CACTGTG，其与第一七聚体间隔33个核苷酸。
We have constructed a library of Balb/c mouse embryo DNA in the vector Charon 4A. The library was searched for sequences homologous to the VH region of a cloned cDNA of the UPC10 heavy chain mRNA. In this paper, we describe the structure and the partial nucleotide sequence of one of such clones (VH441). The nucleotide sequence of this germ-line gene indicates that it encodes amino-acids 1-98 of the X44 and J601 galactan-binding VH regions, but that it differs from the UPC10 VH segment by four single base changes. The VH gene appears to contain a 101 bases long intervening sequence within a precursor sequence identical to the precursor sequence of UPC10. The 3' non coding sequence of the V gene contains the two conserved sequences found in embryonic V DNA segments, CACAGTG and ACATGAACC, separated by 23 nucleotides and a sequence CACTGTG separated by 33 nucleotides from the first heptamer.