New classes of self-cleaving ribozymes revealed by comparative genomics analysis.
New classes of self-cleaving ribozymes revealed by comparative genomics analysis.
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DOI：
10.1038/nchembio.1846
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发表时间：
2015-08
影响因子：
14.8
通讯作者：
Breaker RR
中科院分区：
文献类型：
--
作者：
Weinberg Z;Kim PB;Chen TH;Li S;Harris KA;Lünse CE;Breaker RR
由RNA组成的酶催化蛋白质合成和RNA加工所必需的反应。然而，这样的天然核酶是非常罕见的，这一事实表明，新类别的发现率从20世纪80年代的每年一个下降到每十年一个。事实上，迄今为止，只有11种不同的核酶类被实验验证。最近，我们认识到，自切割核酶经常与某些类型的基因从细菌。在这里，这种同线性被利用来识别两个先前已知的核酶类的不同结构，并发现额外的非编码RNA基序是自切割RNA候选物。三种新的自我切割类，命名为twister sister，pistol和hatchet，已经从这些集合中被鉴定出来，这表明更多的核酶仍然隐藏在现代细胞中。
Enzymes made of RNA catalyze reactions that are essential for protein synthesis and RNA processing. However, such natural ribozymes are exceedingly rare, as evident by the fact that the discovery rate for new classes has dropped to one per decade from about one per year during the 1980s. Indeed, only 11 distinct ribozyme classes have been experimentally validated to date. Recently, we recognized that self-cleaving ribozymes frequently associate with certain types of genes from bacteria. Herein this synteny was exploited to identify divergent architectures for two previously known ribozyme classes and to discover additional noncoding RNA motifs that are self-cleaving RNA candidates. Three new self-cleaving classes, named twister sister, pistol and hatchet, have been identified from this collection, suggesting that even more ribozymes remain hidden in modern cells.