MALDI-TOF mass spectrometric analysis of α-amanitin, β-amanitin, and phalloidin in urine
MALDI-TOF mass spectrometric analysis of α-amanitin, β-amanitin, and phalloidin in urine
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DOI：
10.1007/s11419-012-0145-6
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发表时间：
2012-06
影响因子：
2.2
通讯作者：
K. Gonmori;K. Minakata;Masako Suzuki;I. Yamagishi;H. Nozawa;Koutaro Hasegawa;Amin Wurita;Kanako Watanabe;O. Suzuki
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文献类型：
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作者：
K. Gonmori;K. Minakata;Masako Suzuki;I. Yamagishi;H. Nozawa;Koutaro Hasegawa;Amin Wurita;Kanako Watanabe;O. Suzuki
采用基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）技术建立了一种快速、灵敏的α-鹅膏蕈碱、β-鹅膏蕈碱和鬼笔环肽的分析方法。该方法以α-氰基-4-羟基肉桂酸为基质，辅助毒素的电离。α-鹅膏蕈碱、β-鹅膏蕈碱和鬼笔环肽的钠加合物[M+Na]+atm/z= 941、942和811得到鉴定，微囊藻毒素RR atm/z= 1038得到定量。对于所有毒素，检测限为5 ng/ml，使用0.4 ml尿液，所有校准曲线在10-500 ng/ml范围内呈线性。当使用串联质谱（MS-MS）模式进行检测时，鉴定的灵敏度提高了约10倍。由于这些定量可以在4-200 ng的毒素浓度范围内实现，本MALDI-TOF MS方法可以作为迄今为止报道的用于分析这些蘑菇毒素的最灵敏的方法。据我们所知，本研究是第一次尝试分析鹅膏蕈碱和鬼笔环肽的MALDI-TOF MS（-MS）。
A rapid and sensitive method was developed for analysis of α-amanitin, β-amanitin, and phalloidin by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). In this method, α-cyano-4-hydroxy cinnamic acid was used as the matrix to assist the ionization of toxins. The identification of α-amanitin, β-amanitin, and phalloidin was achieved through their sodium adducts [M+Na]+atm/z= 941, 942, and 811, and quantification of the three toxins was also achieved using microcystin RR atm/z= 1038 as internal standard. For all toxins, the limit of detection was 5 ng/ml, and all calibration curves were linear in the range of 10–500 ng/ml using 0.4 ml of urine. The sensitivity for identification was increased about tenfold when the tandem MS (MS–MS) mode was used for detection. Because these quantifications could be achieved in the toxin concentration range of 4–200 ng, the present MALDI-TOF MS method can serve as the most sensitive method so far reported for the analysis of these mushroom toxins. To our knowledge, this study is the first trial to analyze amanitins and phalloidin by MALDI-TOF MS (-MS).