An acyltransferase-like gene obtained by differential gene expression profiles of quinolizidine alkaloid-producing and nonproducing cultivars of Lupinus angustifolius
An acyltransferase-like gene obtained by differential gene expression profiles of quinolizidine alkaloid-producing and nonproducing cultivars of Lupinus angustifolius
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DOI：
10.5511/plantbiotechnology.10.1109b
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发表时间：
2011-01-01
影响因子：
1.6
通讯作者：
Yamazaki, Mami
中科院分区：
文献类型：
--
作者：
Bunsupa, Somnuk;Okada, Taketo;Yamazaki, Mami
喹诺里西啶生物碱（Quinolizidine alkaloids，QAs）是植物生物碱的代表性类群之一。分离基因参与QA的生物合成，我们进行了差异基因表达分析，通过PCR选择减法之间的QA生产苦品种和非生产甜品种的狭叶羽扇豆。分别获得71个和43个苦味和甜味品种的特异性克隆。在苦味品种中特异性表达的基因中，分离出与BAHD蛋白家族具有同源性的酰基转移酶样基因（LaAT：狭叶羽扇豆酰基转移酶）。LaAT与参与形成共轭多胺的拟南芥BAHD酰基转移酶具有最强的同源性。半定量RT-PCR结果表明，LaAT在苦品种植株的幼叶中表达量最高，但在其它器官中几乎检测不到，而在甜品种中检测不到。
Quinolizidine alkaloids (QAs) are one of the representative groups of plant alkaloids. To isolate genes involved in QA biosynthesis, we performed a differential gene expression analysis by PCR-select subtraction between a QA-producing bitter cultivar and a nonproducing sweet cultivar of Lupinus angustifolius. We obtained 71 and 43 clones specific to the bitter and sweet cultivars, respectively. Among the genes specifically expressed in the bitter cultivar, an acyltransferase-like gene (LaAT: Lupinus angustifolius acyltransferase) showing homology to the BAHD protein family was isolated. LaAT showed the strongest homology to the Arabidopsis thaliana BAHD acyltransferases involved in the formation of conjugated polyamines. Semi-quantitative RT-PCR revealed that LaAT expression was highest in the young leaves but barely detectable in the other organs of the bitter cultivar plant, whereas LaAT expression was undetectable in the sweet cultivar.