Nanopattern-induced changes in morphology and motility of smooth muscle cells
Nanopattern-induced changes in morphology and motility of smooth muscle cells
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DOI：
10.1016/j.biomaterials.2005.01.058
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发表时间：
2005-09-01
期刊：
影响因子：
14
通讯作者：
Leong, KW
中科院分区：
文献类型：
--
作者：
Yim, EKF;Reano, RM;Leong, KW
众所周知，细胞在其自然细胞外环境中被纳米级的地形所包围。研究了牛肺动脉平滑肌细胞(SMC)在聚甲基丙烯酸甲酯(PMMA)和聚二甲基硅氧烷(PDMS)表面的形态、增殖和运动特性。90%以上的细胞排列在栅格上，与培养在非图案化表面的SMC相比，细胞明显延长。细胞核也被拉长和排列在一起。在纳米颗粒表面，细胞的增殖能力显著降低。在体外创伤修复实验中，与细胞迁移相关的微管组织中心(MTOC)的极化在纳米颗粒表面培养的SMC中显示出向细胞轴线倾斜的倾向。相反，非图案化表面上的SMC的MTOC优先偏向伤口边缘。提出这种纳米印迹技术将为研究细胞-基质相互作用和开发具有纳米级特征的医疗设备提供一个有价值的平台。(C)2005爱思唯尔有限公司。保留所有权利。
Cells are known to be surrounded by nanoscale topography in their natural extracellular environment. The cell behavior, including morphology, proliferation, and motility of bovine pulmonary artery smooth muscle cells (SMC) were studied on poly(methyl methacrylate) (PMMA) and poly(dimethylsiloxane) (PDMS) surfaces comprising nanopatterned gratings with 350 nm linewidth, 700nm pitch, and 350nm depth. More than 90% of the cells aligned to the gratings, and were significantly elongated compared to the SMC cultured on non-patterned surfaces. The nuclei were also elongated and aligned. Proliferation of the cells was significantly reduced on the nanopatterned surfaces. The polarization of microtubule organizing centers (MTOC), which are associated with cell migration, of SMC cultured on nanopatterned surfaces showed a preference towards the axis of cell alignment in an in vitro wound healing assay. In contrast, the MTOC of SMC on non-patterned surfaces preferentially polarized towards the wound edge. It is proposed that this nanoimprinting technology will provide a valuable platform for studies in cell-substrate interactions and for development of medical devices with nanoscale features. (c) 2005 Elsevier Ltd. All rights reserved.