Developmental genetics of the perianthless flowers and bracts of a paleoherb species, Saururus chinensis.
Developmental genetics of the perianthless flowers and bracts of a paleoherb species, Saururus chinensis.
复制标题
古草本植物三白草无花被花和苞片的发育遗传学
DOI：
10.1371/journal.pone.0053019
复制
发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Li DZ
中科院分区：
文献类型：
--
作者：
Zhao YH;Larson-Rabin Z;Wang GY;Möller M;Li CY;Zhang JP;Li HT;Li DZ
中国三白草（Saurururus chinensis）是三白草科（Saurururaceae）的核心成员，是一个无花被（没有花瓣或萼片）的科。由于该科植物在系统发育中的基础地位和独特的花部组成，对该科植物的研究对于了解被子植物中无花被花和瓣状苞片的起源和进化具有重要意义。分离与S.以发育中的花序和幼苗叶片为材料，利用抑制性消减杂交（SSH）技术构建花cDNA消减文库。消减cDNA文库包含总共1，141个EST，并用于创建cDNA微阵列以分析发育花序组织的转录谱。随后，qRT-PCR分析的8个MADS-box转录因子和原位杂交的两个B类MADS-box转录因子进行验证和扩展的cDNA微阵列的结果。最后，利用已发现的S. chinensis B类基因的K结构域序列与其他基底被子植物的B基因进行比较。在1,141个基因中，有277个基因在S.其中176个基因至少有一个功能类别，包括转录（14.75%）、能量（12.59%）、代谢（9.12%）、蛋白质相关功能（8.99%）和细胞运输（5.76%）。选择的MADS-box基因的qRT-PCR和原位杂交支持我们的微阵列数据。系统进化分析表明，从S. chinensis。对S. chinensis B-class MADS-box转录因子可能在对着苞片和无花被花的发育过程中起作用。这项研究有助于我们了解三白草属的花序发育，并代表了理解该物种瓣状苞片形成的第一步。
Saururus chinensis is a core member of Saururaceae, a perianthless (lacking petals or sepals) family. Due to its basal phylogenetic position and unusual floral composition, study of this plant family is important for understanding the origin and evolution of perianthless flowers and petaloid bracts among angiosperm species. To isolate genes involved in S. chinensis flower development, subtracted floral cDNA libraries were constructed by using suppression subtractive hybridization (SSH) on transcripts isolated from developing inflorescences and seedling leaves. The subtracted cDNA libraries contained a total of 1,141 ESTs and were used to create cDNA microarrays to analyze transcript profiles of developing inflorescence tissues. Subsequently, qRT-PCR analyses of eight MADS-box transcription factors and in situ hybridizations of two B-class MADS-box transcription factors were performed to verify and extend the cDNA microarray results. Finally, putative phylogenetic relationships within the B-class MADS-box gene family were determined using the discovered S. chinensis B-class genes to compare K-domain sequences with B genes from other basal angiosperms. Two hundred seventy-seven of the 1,141 genes were found to be expressed differentially between S. chinensis inflorescence tissues and seedling leaves, 176 of which were grouped into at least one functional category, including transcription (14.75%), energy (12.59%), metabolism (9.12%), protein-related function (8.99%), and cellular transport (5.76%). qRT-PCR and in situ hybridization of selected MADS-box genes supported our microarray data. Phylogenetic analysis indicated that a total of six B-class MADS-box genes were isolated from S. chinensis. The differential regulation of S. chinensis B-class MADS-box transcription factors likely plays a role during the development of subtending bracts and perianthless flowers. This study contributes to our understanding of inflorescence development in Saururus, and represents an initial step toward understanding the formation of petaloid bracts in this species.
登录
查看更多内容
影响因子：
64.5
作者：
JACK, T;BROCKMAN, LL;MEYEROWITZ, EM
通讯作者：
MEYEROWITZ, EM
影响因子：
11.6
作者：
JOFUKU, KD;DENBOER, BGW;OKAMURO, JK
通讯作者：
OKAMURO, JK
影响因子：
4.1
作者：
Jaramillo, M. Alejandra;Kramer, Elena M.
通讯作者：
Kramer, Elena M.
影响因子：
7.2
作者：
Di Stilio, VS;Kramer, EM;Baum, DA
通讯作者：
Baum, DA
影响因子：
2.9
作者：
Jaramillo, MA;Kramer, EM
通讯作者：
Kramer, EM