Expression of Na(+)-K(+)-ATPase alpha- and beta-subunits along rat nephron: isoform specificity and response to hypokalemia.
Expression of Na(+)-K(+)-ATPase alpha- and beta-subunits along rat nephron: isoform specificity and response to hypokalemia.
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Na( )-K( )-ATPase α 和 β 亚基沿大鼠肾单位的表达：异构体特异性和对低钾血症的反应。
DOI：
10.1152/ajpcell.1994.267.4.c901
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发表时间：
1994
期刊：
影响因子：
--
通讯作者：
Komatsu,Y
中科院分区：
文献类型：
--
作者：
McDonough,AA;Magyar,CE;Komatsu,Y
钠泵(Na(+)-K(+)-腺苷三磷酸酶(Na（+)-K(+)- atp酶）的活性，驱动沿肾元的Na+重吸收活性，根据肾元段变化超过一个数量级，在低钾血症期间外髓收集小管（MCT）的活性增加。根据Barlet-Bas等人（C. Barlet-Bas， E. Arystarkhova， L. Cheval， S. Marsy， K. Sweadner， N. Modyanov和A. Doucet）的建议，本研究的目的是通过免疫印迹法评估大鼠解剖肾单元段中钠泵α 1-和β 1-亚基的丰度，以确定α 2-或α 3蛋白是否可以在收集小管中检测到。生物。兔体内α -1和β -1是否因低钾血症而升高。用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（12-100 mm/道）分离大鼠小管，进行印迹，并用亚单位特异性抗血清进行探针检测。α 1和β 1在所有检测的小管段中检测到，在皮质和髓质厚升肢和近曲小管（PCT）中最高，在MCT中较低，在近直小管中几乎检测不到。在皮质收集小管（CCT）中，α - 1丰度与PCT相当，而β - 1和酶活性都不到PCT的一半。在缺乏K（+）的饮食2周后，MCT中的α - 1和β - 1亚基水平分别增加了3.4 +/- 0.6和11.7 +/- 4.0倍，与活性增加5倍相关。CCT和MCT均未检测到α 2和α 3。（摘要删节250字）
The activity of Na(+)-K(+)-adenosinetriphosphatase (Na(+)-K(+)-ATPase), the sodium pump, which drives active Na+ reabsorption along the nephron, varies over an order of magnitude, depending on the nephron segment, and activity is increased in the outer medullary collecting tubule (MCT) during hypokalemia. The aims of the present study were to assess abundance of sodium pump alpha 1- and beta 1-subunits in dissected nephron segments of the rat by immunoblotting, to determine if alpha 2- or alpha 3-protein could be detected in the collecting tubules, as suggested by Barlet-Bas et al. (C. Barlet-Bas, E. Arystarkhova, L. Cheval, S. Marsy, K. Sweadner, N. Modyanov, and A. Doucet. J. Biol. Chem. 268: 11512-11515, 1993) for rabbit, and to determine if alpha 1 and beta 1 were increased in MCT by hypokalemia. Tubules from the rat were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (12-100 mm/lane), blotted, and probed with subunit-specific antisera. alpha 1 and beta 1, detected in all tubule segments assayed, were highest in cortical and medullary thick ascending limbs and proximal convoluted tubule (PCT), lower in the MCT and barely detectable in proximal straight tubule. In the cortical collecting tubule (CCT), alpha 1 abundance was equivalent to that in PCT, whereas beta 1 and enzymatic activity were both less than one-half of that in PCT. After 2 wk of a K(+)-deficient diet, alpha 1- and beta 1-subunit levels in MCT increased 3.4 +/- 0.6- and 11.7 +/- 4.0-fold, respectively, associated with a 5-fold increase in activity. alpha 2 and alpha 3 were not detected in the CCT or MCT.(ABSTRACT TRUNCATED AT 250 WORDS)
兔皮质集合管中细胞钠诱导的 Na( )-K( )-ATPase 泵的募集是醛固酮依赖性的。
DOI：
10.1016/s0021-9258(19)38450-9
发表时间：
1990
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
M. Blot;F. Wanstok;J. Bonvalet;N. Farman
通讯作者：
N. Farman