Soluble 3-O-sulfated heparan sulfate can trigger herpes simplex virus type 1 entry into resistant Chinese hamster ovary (CHO-K1) cells.
Soluble 3-O-sulfated heparan sulfate can trigger herpes simplex virus type 1 entry into resistant Chinese hamster ovary (CHO-K1) cells.
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DOI：
10.1099/vir.0.82476-0
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发表时间：
2007-04
期刊：
影响因子：
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通讯作者：
V. Tiwari;C. O'donnell;Ronald J. Copeland;Tanya C. Scarlett;Jian Liu;D. Shukla
中科院分区：
文献类型：
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作者：
V. Tiwari;C. O'donnell;Ronald J. Copeland;Tanya C. Scarlett;Jian Liu;D. Shukla
单纯疱疹病毒1型（HSV-1）与糖蛋白D（gD）受体的相互作用促进病毒进入细胞。缺乏细胞受体的中国仓鼠卵巢（CHO-K1）细胞允许病毒附着，但不能进入，这意味着受体在HSV-1感染的附着后（进入）阶段发挥作用。在此，显示了被3-O-磺基转移酶-3（3-OST-3）而不是被3-OST-1修饰的可溶性硫酸乙酰肝素（HS）的存在触发了HSV-1进入抗性CHO-K1细胞。进一步证明，当在3-OST-3-修饰的可溶性HS存在下进行刺入时，糖胺聚糖合成缺陷的CHO-K1突变体变得对进入敏感，表明gD受体的作用是触发进入而不是细胞附着。在单独的实验中，3-OST-3-修饰的可溶性HS也触发了表达HSV-1糖蛋白的细胞与CHO-K1细胞的融合。综上所述，这些结果表明，gD与细胞表面结合受体的缔合对于HSV-1进入和传播不是必需的。
Herpes simplex virus type 1 (HSV-1) interaction with glycoprotein D (gD) receptors facilitates virus entry into cells. Chinese hamster ovary (CHO-K1) cells lacking cellular receptors allow virus to attach, but not to enter, implying a role for receptors during the post-attachment (entry) phase of HSV-1 infection. Here, it is shown that the presence of soluble heparan sulfate (HS) modified by 3-O-sulfotransferase-3 (3-OST-3), but not by 3-OST-1, triggered HSV-1 entry into resistant CHO-K1 cells. It was further demonstrated that a CHO-K1 mutant deficient in glycosaminoglycan synthesis became susceptible to entry when spinoculated in the presence of 3-OST-3-modified soluble HS, indicating that the role of the gD receptor is to trigger entry rather than cell attachment. In separate experiments, 3-OST-3-modified soluble HS also triggered fusion of HSV-1 glycoprotein-expressing cells with CHO-K1 cells. Taken together, these results show that association of gD with cell surface-bound receptor is not essential for HSV-1 entry and spread.