Water-Insoluble Photosensitizer Nanocolloids Stabilized by Supramolecular Interfacial Assembly towards Photodynamic Therapy.
Water-Insoluble Photosensitizer Nanocolloids Stabilized by Supramolecular Interfacial Assembly towards Photodynamic Therapy.
复制标题
通过超分子界面组装稳定水不溶性光敏剂纳米胶体以实现光动力治疗
DOI：
10.1038/srep42978
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发表时间：
2017-02-23
影响因子：
4.6
通讯作者：
Yan X
中科院分区：
文献类型：
--
作者：
Liu Y;Ma K;Jiao T;Xing R;Shen G;Yan X
疏水性光敏剂的纳米工程是改善肿瘤递送和增强光动力治疗（PDT）效率的有前途的方法。通过增强的渗透和滞留（EPR）效应，已经开发了多种用于肿瘤递送PS的递送载体。然而，高度赞赏具有最少使用载体材料且具有优异生物相容性的高性能PS输送系统。本文利用超分子配位的时空界面粘附和组装实现了水不溶性光敏剂二氢卟酚e6（Ce 6）的纳米工程。由于单宁酸（TA）和三价铁（Fe（III））的配位聚合，疏水性Ce 6纳米颗粒通过界面组装膜在水介质中被很好地稳定。所得的Ce 6 @TA-Fe（III）络合物纳米颗粒（称为Ce 6 @TA-Fe（III）NPs）显著提高了载药量（~65%），并且具有60 nm的平均尺寸。Ce 6 @TA-Fe（III）NP作为聚集态几乎不发光，但它们在细胞内内化后可以发光，从而在激光照射下实现低暗毒性和优异的光毒性。与游离载体Ce 6相比，Ce 6 @TA-Fe（III）NP延长了血液循环，促进了PS的肿瘤选择性积累，并增强了体内评价的抗肿瘤功效。
Nanoengineering of hydrophobic photosensitizers (PSs) is a promising approach for improved tumor delivery and enhanced photodynamic therapy (PDT) efficiency. A variety of delivery carriers have been developed for tumor delivery of PSs through the enhanced permeation and retention (EPR) effect. However, a high-performance PS delivery system with minimum use of carrier materials with excellent biocompatibility is highly appreciated. In this work, we utilized the spatiotemporal interfacial adhesion and assembly of supramolecular coordination to achieve the nanoengineering of water-insoluble photosensitizer Chlorin e6 (Ce6). The hydrophobic Ce6 nanoparticles are well stabilized in a aqueous medium by the interfacially-assembled film due to the coordination polymerization of tannic acid (TA) and ferric iron (Fe(III)). The resulting Ce6@TA-Fe(III) complex nanoparticles (referenced as Ce6@TA-Fe(III) NPs) significantly improves the drug loading content (~65%) and have an average size of 60 nm. The Ce6@TA-Fe(III) NPs are almost non-emissive as the aggregated states, but they can light up after intracellular internalization, which thus realizes low dark toxicity and excellent phototoxicity under laser irradiation. The Ce6@TA-Fe(III) NPs prolong blood circulation, promote tumor-selective accumulation of PSs, and enhanced antitumor efficacy in comparison to the free-carrier Ce6in vivoevaluation.