The structural development of primary cultured hippocampal neurons on a graphene substrate
The structural development of primary cultured hippocampal neurons on a graphene substrate
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石墨烯基底上原代培养海马神经元的结构发育
DOI：
10.1016/j.colsurfb.2016.06.045
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发表时间：
2016-10-01
影响因子：
5.8
通讯作者：
Chai, Renjie
中科院分区：
文献类型：
--
作者：
He, Zuhong;Zhang, Shasha;Chai, Renjie
基于石墨烯的纳米材料作为神经修复和再生的神经接口材料的潜力仍然知之甚少。在本研究中，在海马培养模型中确定了神经元对石墨烯底物的反应。结果表明，与商业对照相比，石墨烯基底上的海马培养物的生长和成熟显着改善。具体而言，石墨烯在播种后24小时促进生长锥生长和丝状伪足内部微管的形成，从生长锥中出现的丝状伪足平均数量更高、丝状伪足平均长度更长和生长锥面积更大证明了这一点。石墨烯还显着促进神经突的萌芽和生长。在培养过程中，石墨烯基底上的树突长度、分支点数量和树突复合体指数显着提高。此外，在接种后21天，石墨烯上的树突棘密度增强，树突棘从细到短的成熟得到显着促进。最后，石墨烯显着提高了海马培养物中的突触密度和突触活性。本研究强调了石墨烯作为神经修复和再生的神经接口材料的潜力，并揭示了石墨烯基纳米材料的未来生物医学应用。 (C) 2016 Elsevier B.V. 保留所有权利。
The potential of graphene-based nanomaterials as a neural interfacing material for neural repair and regeneration remains poorly understood. In the present study, the response to the graphene substrate by neurons was determined in a hippocampal culture model. The results revealed the growth and maturation of hippocampal cultures on graphene substrates were significantly improved compared to the commercial control. In details, graphene promoted growth cone growth and microtubule formation inside filopodia 24 h after seeding as evidenced by a higher average number of filopodia emerging from growth cones, a longer average length of filopodia, and a larger growth cone area. Graphene also significantly boosted neurite sprouting and outgrowth. The dendritic length, the number of branch points, and the dendritic complex index were significantly improved on the graphene substrate during culture. Moreover, the spine density was enhanced and the maturation of dendritic spines from thin to stubby spines was significantly promoted on graphene at 21 days after seeding. Lastly, graphene significantly elevated the synapse density and synaptic activity in the hippocampal cultures. The present study highlights graphene's potential as a neural interfacing material for neural repair and regeneration and sheds light on the future biomedical applications of graphene-based nanomaterials. (C) 2016 Elsevier B.V. All rights reserved.