PLGA submicron particles containing chlorhexidine, calcium and phosphorus inhibit Enterococcus faecalis infection and improve the microhardness of dentin
PLGA submicron particles containing chlorhexidine, calcium and phosphorus inhibit Enterococcus faecalis infection and improve the microhardness of dentin
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含氯己定、钙、磷的PLGA亚微米颗粒抑制粪肠球菌感染，提高牙本质显微硬度
DOI：
10.1007/s10856-018-6216-4
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发表时间：
2019-02-01
影响因子：
3.7
通讯作者：
Fan, Bing
中科院分区：
文献类型：
--
作者：
Fan, Wei;Li, Yanyun;Fan, Bing
粪肠球菌 (E. faecalis) 是一种革兰氏阳性兼性厌氧菌，据报道与人类牙齿的大部分难治性根管感染和根管再感染有关。氯己定是一种针对粪肠球菌的强杀菌剂，但不能渗入牙本质小管。另一方面，根管治疗药物常见的负面影响是牙本质显微硬度的降低。在这项研究中，聚（D，L-乳酸-乙交酯）（PLGA）亚微米颗粒被用作递送载体来负载和释放氯己定以及钙和磷。研究了这些颗粒的释放曲线、对粪肠球菌的抗菌能力、牙本质小管的渗透能力、生物相容性以及对牙本质显微硬度的影响。结果表明，封装的化学物质可以以持续的方式从颗粒中释放出来。该颗粒还对 MC3T3-E1 细胞表现出优异的生物相容性，并对粪肠球菌具有显着的抗菌性能。在牙本质切片上，这些颗粒可以通过超声波激活进入牙本质小管并抑制粪肠球菌定植。此外，添加了颗粒的牙本质切片显示出显微硬度的增加。总之，携带氯己定、钙和磷的PLGA亚微米颗粒可以开发成一种用于牙科治疗的新型根管内消毒剂。
Enterococcus faecalis (E. faecalis), a Gram-positive facultative anaerobe, is reported to take responsibility for a large portion of refractory root canal infections and root canal re-infections of human teeth. Chlorhexidine is a strong bactericide against E. faecalis but cannot infiltrate into dentinal tubules. On the other hand, a common negative effect of root canal medicaments is the decrease of dentin microhardness. In this study, poly(D,L-lactic-co-glycolide) (PLGA) submicron particles were applied as delivery carriers to load and release the chlorhexidine as well as calcium and phosphorus. The release profiles, antibacterial ability against E. faecalis, infiltration ability into dentinal tubules, biocompatibility and effects on dentin microhardness of these particles were investigated. Results revealed that encapsulated chemicals could be released in a sustained manner from the particles. The particles also exhibited excellent biocompatibility on MC3T3-E1 cells and significant antimicrobial property against E. faecalis. On dentin slices, the particles could be driven into dentinal tubules by ultrasonic activiation and inhibit E. faecalis colonization. Besides, dentin slices medicated with the particles displayed an increase in microhardness. In conclusion, PLGA submicron particles carrying chlorhexidine, calcium and phosphorus could be developed into a new intra-canal disinfectant for dental treatments.