Retinal pigment epithelial transplants and retinal function in RCS rats.
Retinal pigment epithelial transplants and retinal function in RCS rats.
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发表时间：
1993-10
影响因子：
4.4
通讯作者：
S. Yamamoto;J. Du;P. Gouras;H. Kjeldbye
中科院分区：
文献类型：
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作者：
S. Yamamoto;J. Du;P. Gouras;H. Kjeldbye
目的观察视网膜色素上皮（RPE）移植对皇家外科学院（RCS）品系大鼠视功能的影响。方法12只RCS大鼠于出生后16 ~ 20 d接受RPE移植。移植后3至10个月对视网膜进行电生理和组织学研究，并与11只RCS对照大鼠和11只年龄相当的正常大鼠进行比较。将微电极引导至通过白化病RCS视网膜中的色素沉着可见的移植部位以检测反应。结果所有视网膜均存在自发性神经节细胞活动。在12只移植眼中的9只中检测到神经节细胞对光的反应，但在11只对照眼中的任何一只中都没有检测到。在移植、对照和正常视网膜中分别有96、44和140个单位，其中30%、0%和97%由光驱动。在移植中，分别有36%、29%和28%在移植后3至4个月、6至7个月和10个月驱动。8例移植物中有5例记录了a波和b波的视网膜内ERG。所研究的RCS控制装置均无IERG。移植视网膜的平均IERG为2.5 microV（SD = 1.9），正常视网膜为59 microV（SD = 19）。电极轨迹被追踪到七个视网膜中的六个视网膜的移植部位，这些视网膜对光有反应，并进行组织学检查。结论RCS大鼠视网膜色素上皮细胞移植后能长期维持移植区的视网膜功能。
PURPOSE To determine if retinal pigment epithelium (RPE) transplantation maintains visual function in Royal College of Surgeons (RCS) strain of rats. METHODS Twelve RCS rats received RPE transplants at 16 to 20 days after birth. The retinas were studied electrophysiologically and histologically from 3 to 10 months after transplantation and compared with 11 RCS controls and 11 normal rats of comparable ages. A microelectrode was guided to the transplant site visible by its pigmentation in the albinotic RCS retina to detect responses. RESULTS Spontaneous ganglion cell activity was present in all retinas. Ganglion cell responses to light were detected in 9 of the 12 transplant eyes but not in any of the 11 controls. 96, 44, 140 units were encountered and 30%, 0%, 97% were driven by light respectively in transplant, control, and normal retinas. In transplants 36%, 29%, and 28% were driven at 3 to 4, 6 to 7, and 10 months after transplantation, respectively. Intraretinal ERGs with both a- and b-waves were recorded in 5 of the 8 transplants studied. None of the RCS controls studied had an IERG. The average IERG was 2.5 microV (SD = 1.9) in transplants and 59 microV (SD = 19) in normal retinas. The electrode track was traced to the transplant site in six of the seven retinas that were responsive to light and examined histologically. CONCLUSION RPE transplants to RCS rats maintain retinal function in the transplant site for long periods of time.