Identification of a new quaternary neutralizing epitope on human immunodeficiency virus type 1 virus particles
Identification of a new quaternary neutralizing epitope on human immunodeficiency virus type 1 virus particles
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DOI：
10.1128/jvi.79.8.5232-5237.2005
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发表时间：
2005-04-01
影响因子：
5.4
通讯作者：
Zolla-Pazner, S
中科院分区：
文献类型：
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作者：
Gorny, MK;Stamatatos, L;Zolla-Pazner, S
通过结合试验选择人类免疫缺陷病毒（HIV） I型特异性人单克隆抗体（mab）可能无法识别抗体与完整病毒粒子上的四级表位。HIV中和试验用于选择人单抗2909，它能有效中和SF162，并识别病毒表面的表位，但不能识别可溶性蛋白。gp120的三个区域，V2和V3环以及CD4结合域，参与了MAb 2909识别的表位。这种独特的单克隆抗体的存在，定义了一个由四级结构形成的复杂表位，表明可能存在其他新的中和性HIV表位作为疫苗的靶点。
The selection of human monoclonal antibodies (MAbs) specific for human immunodeficiency virus (HIV) type I by binding assays may fail to identify Abs to quaternary epitopes on the intact virions. The HIV neutralization assay was used for the selection of human MAb 2909, which potently neutralizes SF162 and recognizes an epitope on the virus surface but not on soluble proteins. Three regions of gp120, the V2 and V3 loops and the CD4 binding domain, contribute to the epitope recognized by MAb 2909. The existence of such a unique MAb, which defines a complex epitope formed by a quaternary structure, suggests that there may be other new neutralizing HIV epitopes to target with vaccines.