BMAL1 regulates mitochondrial fission and mitophagy through mitochondrial protein BNIP3 and is critical in the development of dilated cardiomyopathy
BMAL1 regulates mitochondrial fission and mitophagy through mitochondrial protein BNIP3 and is critical in the development of dilated cardiomyopathy
复制标题
BMAL1 通过线粒体蛋白 BNIP3 调节线粒体裂变和线粒体自噬，在扩张型心肌病的发展中至关重要
DOI：
10.1007/s13238-020-00713-x
复制
发表时间：
2020-04-10
期刊：
影响因子：
21.1
通讯作者：
Sun, Ning
中科院分区：
文献类型：
--
作者：
Li, Ermin;Li, Xiuya;Sun, Ning
Dysregulation of circadian rhythms associates with cardiovascular disorders. It is known that deletion of the core circadian geneBmal1in mice causes dilated cardiomyopathy. However, the biological rhythm regulation system in mouse is very different from that of humans. WhetherBMAL1plays a role in regulating human heart function remains unclear. Here we generated aBMAL1knockout human embryonic stem cell (hESC) model and further derived humanBMAL1deficient cardiomyocytes. We show thatBMAL1deficient hESC-derived cardiomyocytes exhibited typical phenotypes of dilated cardiomyopathy including attenuated contractility, calcium dysregulation, and disorganized myofilaments. In addition, mitochondrial fission and mitophagy were suppressed inBMAL1deficient hESC-cardiomyocytes, which resulted in significantly attenuated mitochondrial oxidative phosphorylation and compromised cardiomyocyte function. We also found that BMAL1 binds to the E-box element in the promoter region ofBNIP3gene and specifically controls BNIP3 protein expression.BMAL1knockout directly reduced BNIP3 protein level, causing compromised mitophagy and mitochondria dysfunction and thereby leading to compromised cardiomyocyte function. Our data indicated that the core circadian geneBMAL1is critical for normal mitochondria activities and cardiac function. Circadian rhythm disruption may directly link to compromised heart function and dilated cardiomyopathy in humans.
