Conventional, but not remote ischemic preconditioning, reduces iNOS transcription in liver ischemia/reperfusion
Conventional, but not remote ischemic preconditioning, reduces iNOS transcription in liver ischemia/reperfusion
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DOI：
10.3748/wjg.v20.i28.9506
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发表时间：
2014-07-28
影响因子：
4.3
通讯作者：
Sandstrom, Per
中科院分区：
文献类型：
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作者：
Bjornsson, Bergthor;Winbladh, Anders;Sandstrom, Per
目的：目的：探讨预处理对大鼠肝缺血再灌注损伤（IRI）诱导型一氧化氮合酶（iNOS）和白细胞介素1（IL-1）受体转录的影响。节段性缺血1h缺血预处理（IPC）组和远程缺血预处理（R-IPC）组。IPC和R-IPC均为缺血10 min再灌注10 min。采用真实的时间PCR检测肝组织中iNOS和IL-1受体mRNA的表达。分析来自肝脏的连续采样微透析液（MD）中的总亚硝酸盐和硝酸盐（NOx）。结果：再灌注4 h后，R-IPC组iNOSmRNA（Δ Ct：3.44 ± 0.57）明显高于IPC组（Δ Ct：5.86 ± 0.82）（P = 0.025）;在再灌注期间，IPC组中IL-1受体转录活性降低（Δ Ct：1.88 +/- 0.53至4.81 +/- 0.21），但R-IPC组中没有（P = 0.027）。在MD中，与缺血早期的水平相比，缺血结束时R-IPC组的NOx水平显著下降（12.3 +/- 2.2至4.7 +/- 1.2 μ mol/L）（P = 0.008）。IPC组也观察到类似的趋势（11.8 +/- 2.1至6.4 +/- 1.5 μ mol/L），尽管这种差异在统计学上不显着。结论：IPC能降低再灌注早期iNOS和IL-1受体的转录，提示缺血再灌注后炎症反应较轻，而R-IPC无此作用。NOx在缺血的肝叶中被消耗。(C)2014百世登出版集团股份有限公司All rights reserved.
AIM: To study the effects of preconditioning on inducible nitric oxide synthase (iNOS) and interleukin 1 (IL-1) receptor transcription in rat liver ischemia/reperfusion injury (IRI).METHODS: Seventy-two male rats were randomized into 3 groups: the one-hour segmental ischemia (IRI, n = 24) group, the ischemic preconditioning (IPC, n = 24) group or the remote ischemic preconditioning (R-IPC, n = 24) group. The IPC and R-IPC were performed as 10 min of ischemia and 10 min of reperfusion. The iNOS and the IL-1 receptor mRNA in the liver tissue was analyzed with real time PCR. The total Nitrite and Nitrate (NOx) in continuously sampled microdialysate (MD) from the liver was analyzed. In addition, the NOx levels in the serum were analyzed.RESULTS: After 4 h of reperfusion, the iNOS mRNA was significantly higher in the R-IPC (Delta Ct: 3.44 +/- 0.57) group than in the IPC (Delta Ct: 5.86 +/- 0.82) group (P = 0.025). The IL-1 receptor transcription activity was reduced in the IPC group (Delta Ct: 1.88 +/- 0.53 to 4.81 +/- 0.21), but not in the R-IPC group, during reperfusion (P = 0.027). In the MD, a significant drop in the NOx levels was noted in the R-IPC group (12.3 +/- 2.2 to 4.7 +/- 1.2 mu mol/L) at the end of ischemia compared with the levels in early ischemia (P = 0.008). A similar trend was observed in the IPC group (11.8 +/- 2.1 to 6.4 +/- 1.5 mu mol/L), although this difference was not statistically significant. The levels of NOx rose quickly during reperfusion in both groups.CONCLUSION: IPC, but not R-IPC, reduces iNOS and IL-1 receptor transcription during early reperfusion, indicating a lower inflammatory reaction. NOx is consumed in the ischemic liver lobe. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.