Cucurbitacin D exhibits its anti-cancer effect in human breast cancer cells by inhibiting Stat3 and Akt signaling
Cucurbitacin D exhibits its anti-cancer effect in human breast cancer cells by inhibiting Stat3 and Akt signaling
复制标题
DOI：
10.1177/1721727x17751809
复制
发表时间：
2018-01-30
影响因子：
0.7
通讯作者：
Ko, Seong-Gyu
中科院分区：
文献类型：
--
作者：
Ku, Jin Mo;Hong, Se Hyang;Ko, Seong-Gyu
葫芦素是葫芦科和十字花科中常见的三萜类化合物，在传统医学中应用已久。葫芦素具有抗炎和抗癌作用。我们研究了葫芦素D是否影响乳腺癌细胞的生存能力及其作用机制。采用MTT法测定乳腺癌细胞的活力。Western blot检测多种调节因子如p-p53、p-Stat3、p-Akt和p-NF-kappa b的表达，阿霉素和葫芦素D影响MCF7、mda - mb - 23i和SKBR3细胞的活力。葫芦素D和阿霉素增加了MCF7、SKBR3和MDA-MB-231细胞中p-p53的表达。葫芦素D抑制MCF7、mda - mb - 23i和SKBR3细胞中p-Akt、p-NF-kappa B和p-Stat3的表达。单用阿霉素不降低p-Akt和p-Stat3水平。葫芦素D降低p- nf - κ B和p-Stat3水平。阿霉素联合葫芦素D比单独使用葫芦素D更能提高p-p53水平，抑制Akt、nf - κ B、Stat3和Bcl-2的表达。我们的研究结果清楚地表明，葫芦素D可能是一种治疗人类乳腺癌的有用化合物。
Cucurbitacins are triterpenoids commonly found in Cucurbitaceae and Cruciferae and have long been used in traditional medicine. Cucurbitacins demonstrate anti-inflammatory and anti-cancer activities. We investigated whether cucurbitacin D affects viability in breast cancer cells and its mechanism of action. An MTT assay was used to measure the viability of breast cancer cells. Western blot analysis was used to measure the expression of various modulators, such as p-p53, p-Stat3, p-Akt, and p-NF-kappa B. Doxorubicin and cucurbitacin D affected the viability of MCF7, MDA-MB-23 I , and SKBR3 cells. Cucurbitacin D and doxorubicin increased p-p53 expression in MCF7, SKBR3, and MDA-MB-231 cells. Cucurbitacin D suppressed p-Akt, p-NF-kappa B, and p-Stat3 expression in MCF7, MDA-MB-23 I, and SKBR3 cells. Doxorubicin alone did not decrease p-Akt and p-Stat3 levels. Cucurbitacin D decreased p-NF-kappa B and p-Stat3 levels. Doxorubicin in combination with cucurbitacin D increased p-p53 levels and suppressed Akt, NF-kappa B, Stat3, and Bcl-2 expression more than cucurbitacin D alone. Our results clearly demonstrate that cucurbitacin D could be a useful compound for treating human breast cancer.