GLYCOSAMINOGLYCAN TURN-OVER IN ARTICULAR-CARTILAGE
GLYCOSAMINOGLYCAN TURN-OVER IN ARTICULAR-CARTILAGE
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DOI：
10.1098/rstb.1975.0054
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发表时间：
1975-01-01
期刊：
影响因子：
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通讯作者：
STOCKWELL, RA
中科院分区：
文献类型：
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作者：
MAROUDAS, A;LEABACK, DH;STOCKWELL, RA
使用[35S]硫酸钠作为前体，对糖胺聚糖的周转进行了体内和体外研究。对兔子和狗进行体内实验，在整个实验过程中特别注意监测血清中的35S放射性，并在每次实验结束时测量由于软骨中未掺入的无机[35S]硫酸盐引起的放射性，以及由于掺入的硫酸盐引起的放射性。还测定了血清的无机硫酸盐含量以及软骨和血清之间无机硫酸盐离子的分配系数。根据这些信息，可以准确计算体内软骨吸收硫酸盐的速率，从而计算周转率。然后对兔和狗的软骨进行了体外实验，并对体内和体外结果进行了比较。两组结果之间获得了非常好的一致性。然后在完全相同的体外条件下对人体关节软骨进行研究，从而可以获得可以相信接近实际体内值的后者的周转率。由此获得的平均半衰期从幼兔的 45 天到成年狗的 150 天和人股骨头的 800 天不等。在人类软骨中，单个关节内的周转率作为表面以下深度的函数以及不同关节之间存在相当大的变化。因此，人类股骨头的平均半衰期为 800 天，而股骨髁的平均半衰期为 300 天。尽管周转率稍高，但骨关节股骨头的软骨在硫酸盐吸收方面与正常标本似乎没有太大差异。
Glycosaminoglycan turn-over has been studied bothin vivoandin vitro, by using sodium [35S]sulphate as a precursor. Thein vivoexperiments were performed on rabbits and dogs, taking special care to monitor the35S radioactivity in the serum throughout the experiment and to measure the radioactivity due to unincorporated inorganic [35S] sulphate in cartilage at the end of each experiment, in addition to that due to incorporated sulphate. The inorganic sulphate content of the serum was also determined as well as the distribution coefficient for the inorganic sulphate ion between cartilage and serum. From this information it was possible to calculate accurately the rate of sulphate uptake by cartilagein vivoand hence the turn-over rate. Experiments were then performedin vitroon cartilage from rabbits and dogs and thein vivoandin vitroresults were compared. A very good agreement was obtained between the two sets of results. Studies were then carried out under exactly the samein vitroconditions on human articular cartilage and it was thus possible to obtain a turn-over rate for the latter which one could trust was close to the actualin vivovalue. The mean half-lives thus obtained varied from 45 days for the young rabbit to 150 days for the adult dog and 800 days for the human femoral head. In human cartilage there were considerable variations in turn-over rate within a single joint as a function of depth below the surface, and between different joints. Thus, while the mean half-life for the human femoral head is 800 days, that for the femoral condyle is 300 days. Cartilage from osteoarthrosic femoral heads did not appear to differ much with respect to sulphate uptake from the normal specimens although the turn-over rates were somewhat higher.