Comparative Ocular Microbial Communities in Humans with and without Blepharitis
Comparative Ocular Microbial Communities in Humans with and without Blepharitis
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DOI：
10.1167/iovs.12-9922
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发表时间：
2012-08-01
影响因子：
4.4
通讯作者：
Jeon, Che Ok
中科院分区：
文献类型：
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作者：
Lee, Se Hee;Oh, Doo Hwan;Jeon, Che Ok
目的。方法：采用焦磷酸测序方法对7例眼睑炎患者和4例健康人的睫毛和泪液标本进行细菌16S rRNA基因测序，并对其细菌群落结构进行生物信息学比较。结果系统学分析表明，睫毛和泪液样本中细菌群落高度多样化，含有许多未被描述的细菌。睫毛炎患者睫毛中细菌群落多样性低于健康对照组，而泪液中细菌群落多样性高于健康对照组。用UniFrac和主坐标分析进行统计分析表明，无论个体如何，睑炎患者泪液样本的细菌群落都很好地聚集在一起，而所有睫毛样本和健康泪液样本的细菌群落由于高度的人际变异性而没有很好地聚集在一起。生物信息学分析表明，丙酸杆菌、葡萄球菌、链霉菌、棒状杆菌和芽孢杆菌是常见的眼部细菌。从相对丰度来看，葡萄球菌、链球菌属、棒状杆菌和芽孢杆菌的相对丰度增加，丙酸杆菌的相对丰度减少。结论：眼睑炎组中链球菌、棒状杆菌和芽孢杆菌的相对丰度较高，提示人类眼缘炎可能是由花粉、粉尘和土壤颗粒的侵袭引起的。这些结果将为以眼部微生物区系为基础的人类眼缘炎的防治提供有价值的信息。(投资眼科VS科学。2012年；5355855593)doi：10.1167/iovs.12-9922.
PURPOSE. The aims of our study were to compare the ocular microbial communities of humans with and without blepharitis in an attempt to elucidate which microorganisms may cause blepharitis.METHODS. Bacterial 16S rRNA genes of eyelash and tear samples from seven blepharitis patients and four healthy controls were sequenced using a pyrosequencing method, and their bacterial community structures were compared bioinformatically.RESULTS. Phylotypic analysis demonstrated that eyelash and tear samples had highly diverse bacterial communities with many previously undescribed bacteria. Bacterial communities in eyelash samples from subjects with blepharitis were less diverse than those from healthy controls, while the bacterial communities of tear subjects with blepharitis were more diverse than those of healthy subjects. Statistical analyses using UniFrac and a principle coordinate analysis showed that the bacterial communities of tear samples from subjects with blepharitis were well clustered, regardless of individual, while the bacterial communities of all eyelash samples and healthy tear samples were not well clustered due to high interpersonal variability. Bioinformatic analysis revealed that Propionibacterium, Staphylococcus, Streptophyta, Corynebacterium, and Enhydrobacter were the common ocular bacteria. An increase of Staphylococcus, Streptophyta, Corynebacterium, and Enhydrobacter, and a decrease of Propionibacterium were observed from blepharitis subjects, in terms of the relative abundances.CONCLUSIONS. Higher abundances of Streptophyta, Corynebacterium, and Enhydrobacter in blepharitis subjects suggested that human blepharitis might be induced by the infestations of pollens, dusts, and soil particles. These results will provide valuable information for the prevention and treatment of human blepharitis based on ocular microbial flora. (Invest Ophthalmol Vis Sci. 2012;53:5585-5593) DOI:10.1167/iovs.12-9922