Bio-inorganic speciation analysis by hyphenated techniques.
Bio-inorganic speciation analysis by hyphenated techniques.
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DOI：
10.1039/a909137h
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
J. Szpunar
中科院分区：
文献类型：
--
作者：
J. Szpunar
本文综述了色谱和电泳分离技术结合元素选择性检测对生物样品中痕量金属和类金属进行种选择性分析的研究进展。感兴趣的物种包括有机砷，有机硒和金属络合物与生物配体，如植物螯合素，金属硫蛋白，蛋白质和多糖。讨论的分离机制包括尺寸排阻，阴离子和阳离子交换和反相HPLC和平板和毛细管区带电泳。各种元素选择性（例如，AAS、ICP-AES和ICP-MS）和分子特异性（电喷雾MS/MS）检测技术进行了讨论。以表格形式全面综述了耦合技术在生物材料分析中的应用。重点讨论了生物无机形态分析中的样品制备和误差来源。
The state-of-the-art of species-selective analysis for trace metals and metalloids in biological materials by chromatographic and electrophoretic separation techniques with element selective detection is critically reviewed. The species of interest include organoarsenic, organoselenium and metal complexes with bioligands such as phytochelatins, metallothioneins, proteins and polysaccharides. The separation mechanisms discussed include size-exclusion, anion- and cation-exchange and reversed-phase HPLC and flatbed and capillary zone electrophoresis. Advantages and limitations of various element selective (e.g., AAS, ICP-AES and ICP-MS) and molecule specific (electrospray MS/MS) detection techniques used on- and off-line are discussed. The applications of coupled techniques to the analysis of biological materials are comprehensively reviewed in tabular form. Attention is paid to the sample preparation and sources of error in bioinorganic speciation analysis.