Identification and isolation of a novel herpesvirus in a captive mob of eastern grey kangaroos (Macropus giganteus)
Identification and isolation of a novel herpesvirus in a captive mob of eastern grey kangaroos (Macropus giganteus)
复制标题
DOI：
10.1016/j.vetmic.2007.11.019
复制
发表时间：
2008-06-22
影响因子：
3.3
通讯作者：
Terio, Karen A.
中科院分区：
文献类型：
--
作者：
Smith, Joseph A.;Wellehan, James F. X., Jr.;Terio, Karen A.
在对溃疡性泄殖腔炎患者进行诊断性检查期间，在一群圈养的东部灰袋鼠（Macropus kangaroos）中检测到一种新型疱疹病毒。最初使用共有疱疹病毒PCR在组织中检测到病毒。泄殖腔病变的常规组织学或透射电子显微镜切片中没有明显的病毒包涵体或颗粒。从样本中分离出病毒，并对所得分离株进行透射电子显微镜检查，证实该病毒在形态上与疱疹病毒一致。从组织样本和分离株的PCR产物的核苷酸测序显示，该病毒是在亚科γ疱疹病毒，是不同于其他已知的疱疹病毒。病变和新病毒之间的相关性仍然未知。两种疱疹病毒，都属于α疱疹病毒亚科，先前已在大足类动物中描述过，并且已知会引起全身性临床疾病。这是第一次报告的γ疱疹病毒的目的有袋目，并可能提供有价值的信息，关于这个病毒家族的进化和繁殖。根据目前疱疹病毒的命名规则，作者建议将这种新的疱疹病毒命名为Macropodid herpesvirus 3（MaHV-3）。(C)2007 Elsevier B. V.保留所有权利。
A novel herpesvirus was detected in a captive mob of eastern grey kangaroos (Macropus giganteus) during diagnostic workup for individuals with ulcerative cloacitis. Virus was initially detected in tissues using a consensus herpesvirus PCR. No viral inclusions or particles had been evident in routine histologic or transmission electron microscopic sections of cloacal lesions. Virus was isolated from samples and transmission electron microscopy of the resulting isolates confirmed that the virus was morphologically consistent with a herpesvirus. Nucleotide sequencing of the PCR product from tissue samples and from the isolates revealed that the virus was in the subfamily Gammaherpesvirinae and was distinct from other known herpesviruses. The correlation between the lesions and the novel virus remains unknown. Two herpesviruses, both in the subfamily Alphaherpesvirinae, have previously been described in macropods and are known to cause systemic clinical disease. This is the first reported gammaherpesvirus within the order Marsupialia, and may provide valuable information regarding the evolution and phylogeny of this virus family. Based on current herpesvirus nomenclature convention, the authors propose the novel herpesvirus be named Macropodid herpesvirus 3 (MaHV-3). (C) 2007 Elsevier B.V. All rights reserved.