Engineering porcine arteries: effects of scaffold modification.
Engineering porcine arteries: effects of scaffold modification.
复制标题
工程猪动脉：支架修饰的影响。
DOI：
10.1002/jbm.a.10603
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Niklason,LauraE
中科院分区：
文献类型：
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作者：
Prabhakar,Vikas;Grinstaff,MarkW;Alarcon,Javier;Knors,Chris;Solan,AmyK;Niklason,LauraE
已经开发了在聚乙醇酸（PGA）支架上培养牛或猪血管细胞以形成工程化血管的技术。以前，它表明，平滑肌细胞（SMC），在接近PGA残留物培养8周后，有较低的表达SMC的分化标志物和有丝分裂的SMC相比，远离聚合物残留物。探讨了PGA的修饰作为使培养后残留的聚合物片段最小化的手段。为了加速降解，聚合物用热、NaOH或γ辐射处理。差示扫描量热法、质量和拉伸强度降解以及特性粘度用于评估聚合物特性。当聚合物保持在水性条件下时，对于每次处理，经处理的PGA的拉伸强度在3周内降解至零。在NaOH和γ-处理的聚合物上培养的工程化血管构建体显示出整个血管壁的平滑肌α-肌动蛋白。支架处理影响移植物形态、细胞分化和机械完整性。© 2003 Wiley Periodicals，Inc. J Biomed Mater Res 67A：303-311，2003
Techniques have been developed to culture bovine or porcine vascular cells on polyglycolic acid (PGA) scaffolds to form engineered vessels. Previously, it was shown that smooth muscle cells (SMCs) that were in close proximity to PGA remnants after 8 weeks of culture had lower expression of SMC markers of differentiation and were more mitotic compared with SMCs that were distant from polymer residuals. Modifications of PGA were explored as a means to minimize residual polymer fragments after culture. To hasten degradation, polymer was treated with heat, NaOH, or γ‐irradiation. Differential scanning calorimetry, mass and tensile strength degradation, and inherent viscosity were used to assess polymer characteristics. When polymer was maintained in aqueous conditions, tensile strength of treated PGA degraded to zero within 3 weeks for each treatment. Engineered vessel constructs cultured on NaOH and γ‐treated polymer displayed smooth muscle α‐actin throughout the vessel wall. Scaffold treatment impacted graft morphology, cellular differentiation, and mechanical integrity. © 2003 Wiley Periodicals, Inc. J Biomed Mater Res 67A: 303–311, 2003