Toxic effects of bisphenol A on goldfish gonad development and the possible pathway of BPA disturbance in female and male fish reproduction
Toxic effects of bisphenol A on goldfish gonad development and the possible pathway of BPA disturbance in female and male fish reproduction
复制标题
双酚A对金鱼性腺发育的毒性作用及BPA干扰雌雄鱼繁殖的可能途径
DOI：
10.1016/j.chemosphere.2019.01.033
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发表时间：
2019-04-01
期刊：
影响因子：
8.8
通讯作者：
Li, Yuanyou
中科院分区：
文献类型：
--
作者：
Wang, Qing;Yang, Huirong;Li, Yuanyou
双酚 A (BPA) 是一种存在于水生环境中的丰富的内分泌干扰化合物，对鱼类繁殖产生不利影响；然而，这些影响的确切途径尚不清楚。在本研究中，研究了 BPA 对金鱼（Carassius auratus）卵巢和睾丸发育的不同影响以及这些影响背后的不同机制。性腺体指数（GSI）和性腺组织学表明，BPA 会降低金鱼的卵巢成熟度，但在停止 BPA 治疗后，卵巢成熟度会恢复。在男性中，BPA 会破坏睾丸成熟，但这种破坏在 BPA 治疗停止后无法恢复。在BPA处理的雌鱼中，下丘脑-垂体-性腺（HPG）轴相关基因sgnrh、fsh beta和lh beta显着降低，而卵巢中性类固醇激素水平没有变化，也没有发现TUNEL和PCNA染色，表明BPA可能通过HPG轴减少卵巢成熟。在雄性鱼中，在暴露于1μg L-1 BPA的生殖细胞和暴露于50和500μg L-1 BPA的睾丸细胞中发现TUNEL染色，在暴露于50和500μg L-1 SPA的鱼中也发现11-KT水平降低，但BPA不影响与HPG轴相关的基因。该结果表明，BPA 通过生殖细胞和 Leydig 细胞凋亡来破坏睾丸成熟，从而诱导 11-KT 水平下降，从而破坏精子发生。总的来说，我们的研究结果为了解 BPA 干扰金鱼繁殖的分子和细胞机制提供了见解。 (C) 2019 Elsevier Ltd. 保留所有权利。
Bisphenol A (BPA) is an abundant endocrine-disrupting compound that is found in the aquatic environment and has adverse effects on fish reproduction; however, the exact pathway of these impacts is unclear. In this study, the different effects of BPA on ovarian and testis development in goldfish (Carassius auratus) and the different mechanisms underlying these effects were investigated. The gonadosomatic index (GSI) and gonadal histology demonstrated that BPA diminished ovarian maturation in goldfish, which recovered after BPA treatment withdrawal. In males, BPA disrupted testis maturation, but this disruption could not be recovered after BPA treatment withdrawal. The hypothalamic-pituitary-gonad (HPG) axis-related genes sgnrh, fsh beta and lh beta were significantly decreased in BPA-treated female fish, while no changes in sex steroid hormone levels and no TUNEL and PCNA staining were found in the ovary, suggesting that BPA may reduce ovarian maturation through the HPG axis. In male fish, TUNEL staining was found in 1 mu g L-1 BPA-exposed germ cells and 50 and 500 mu g L-1 BPA-exposed Leydig cells, Decreases in 11-KT levels were also found in 50 and 500 mu g L-1 SPA-exposed fish, but BPA did not affect genes associated with the HPG axes. This result shows that BPA disrupts testis maturation through apoptosis of germ cells and Leydig cells, thus inducing decreases in 11-KT levels that disrupt spermatogenesis. Collectively, our findings provide insights into the molecular and cellular mechanisms underlying BPA disturbance of goldfish reproduction. (C) 2019 Elsevier Ltd. All rights reserved.