A moth odorant receptor highly expressed in the ovipositor is involved in detecting host-plant volatiles
A moth odorant receptor highly expressed in the ovipositor is involved in detecting host-plant volatiles
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在产卵器中高度表达的蛾气味受体参与检测寄主植物挥发物
DOI：
10.7554/elife.53706
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发表时间：
2020-05-21
期刊：
影响因子：
7.7
通讯作者：
Wang, Chen-Zhu
中科院分区：
文献类型：
--
作者：
Li, Rui-Ting;Huang, Ling-Qiao;Wang, Chen-Zhu
触角通常被认为是昆虫的鼻孔。在这里，我们对棉毛螺螺虫信息素腺体-产卵器复合物的转录组进行了测序，发现气味受体（OR）基因HassOR31在产卵器中的表达比在触角或其他组织中的表达高得多。为了确定促排卵剂是否参与气味检测，我们在爪蟾卵母细胞模型系统中共表达HassOR31及其共受体HassORco，并证明OR对12种植物气味剂有反应，特别是z -3-己烯丁酸酯。这些气味引起了产卵者部分感受器的电生理反应，HassOR31和HassORco在产卵者感受器中共表达。两次产卵偏好实验表明，缺乏触角的雌蛾仍然优先选择含有植物挥发物的产卵地点。我们认为，HassOR31在沙刺黄蚕产卵器中的表达有助于雌性在寄主植物中确定精确的产卵位置。
Antennae are often considered to be the nostrils of insects. Here, we sequenced the transcriptome of the pheromone gland-ovipositor complex of Helicoverpa assulta and discovered that an odorant receptor (OR) gene, HassOR31, had much higher expression in the ovipositor than in antennae or other tissues. To determine whether the ovipositor was involved in odorant detection, we co-expressed HassOR31 and its co-receptor, HassORco, in a Xenopus oocyte model system, and demonstrated that the OR was responsive to 12 plant odorants, especially Z-3-hexenyl butyrate. These odorants elicited electrophysiological responses of some sensilla in the ovipositor, and HassOR31 and HassORco were co-expressed within ovipositor sensilla. Two oviposition preference experiments showed that female moths lacking antennae still preferentially selected oviposition sites containing plant volatiles. We suggest that the expression of HassOR31 in the ovipositor of H. assulta helps females to determine precise egg-laying sites in host plants.