A genetic linkage map of Brassica carinata constructed with a doubled haploid population
A genetic linkage map of Brassica carinata constructed with a doubled haploid population
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双单倍体群体构建的甘蓝遗传连锁图
DOI：
10.1007/s00122-012-1898-3
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发表时间：
2012-10-01
影响因子：
5.4
通讯作者：
Meng, Jinling
中科院分区：
文献类型：
--
作者：
Guo, Shaomin;Zou, Jun;Meng, Jinling
十字花型油菜是一种重要的油料作物，具有独特的优势，值得其他油菜作物借鉴。然而，鉴于对龙须草遗传资源的研究有限，对该物种的遗传结构的了解相对较少。通过小孢子培养获得了9份纯合的、遗传差异较大的红花甜菜材料，利用其中的2个发散双单倍体材料构建了一个由183个单倍体材料组成的双单倍体图谱群体。作图群体显示了多个感兴趣性状的分离。构建了甘蓝型油菜B基因组8个连锁群和C基因组9个连锁群的遗传图谱，该图谱覆盖1 703 cM，共212个座位，与S等其他重要芥菜属植物的遗传图谱进行了比较。利用该连锁图谱确定了与色素沉着相关的两个孟德尔遗传性状(花瓣和花药顶色)和一个数量性状(种皮颜色)的基因座。讨论了作图群体在十字花科作物遗传改良中的意义。
Brassica carinata is an important oilseed crop with unique favourable traits that are desirable for other Brassica crops. However, given the limited research into genetic resources in B. carinata, knowledge of the genetic structure of this species is relatively poor. Nine homozygous, genetically distinct accessions of B. carinata were obtained via microspore culture, from which two divergent doubled haploid (DH) lines were used to develop a DH mapping population that consisted of 183 lines. The mapping population showed segregation of multiple traits of interest. A genetic map was constructed with PCR-based markers, and a total of 212 loci, which covered 1,703 cM, were assigned to eight linkage groups in the B genome and nine linkage groups in the C genome, which allowed comparison with genetic maps of other important Brassica species that contain the B/C genome(s). Loci for two Mendelian-inherited traits related to pigmentation (petal and anther tip colour) and one quantitative trait (seed coat colour) were identified using the linkage map. The significance of the mapping population in the context of genetic improvement of Brassica crops is discussed.