Identification of meat and poultry species in food products using DNA barcoding
Identification of meat and poultry species in food products using DNA barcoding
复制标题
DOI：
10.1016/j.foodcont.2017.04.025
复制
发表时间：
2017-10-01
期刊：
影响因子：
6
通讯作者：
Hernandez, Eduardo L.
中科院分区：
文献类型：
--
作者：
Hellberg, Rosalee S.;Hernandez, Brenda C.;Hernandez, Eduardo L.
DNA条形码是一种基于测序的食品中肉类和家禽物种鉴定的有前途的方法。然而，加工过程中的DNA降解可能会限制从这些食品中回收全长DNA条形码。本研究的目的是调查DNA条形码识别肉类和家禽产品中物种的能力，并比较全长条形码（658 bp）和迷你条形码（127 bp）的结果。本研究收集了60种肉类和家禽产品，包括熟肉、碎肉、肉干和罐头肉。每个样品经历细胞色素c氧化酶亚基I（COI）基因的完整和微型条形码化。将所得序列针对生命条形码数据库（BOLD）和GenBank查询以用于物种鉴定。总体而言，与微型条形码（38.3%）相比，全条形码显示出更高的测序成功率（68.3%）。微型条形码优于完整条形码，用于罐头产品的鉴定（23.8%对19.0%成功），以及火鸡和鸭肉产品;然而，当对鸡肉、牛肉和野牛/布法罗进行测试时，引物组表现不佳。总体而言，完整的条形码被认为是检测肉类和家禽产品中物种的可靠方法，罐头产品除外。微型条形码显示出用于加工产品中的物种鉴定的高潜力;然而，该应用需要改进的引物组。(C)2017爱思唯尔有限公司版权所有。
DNA barcoding is a promising method for the sequencing-based identification of meat and poultry species in food products. However, DNA degradation during processing may limit recovery of the full-length DNA barcode from these foods. The objective of this study was to investigate the ability of DNA barcoding to identify species in meat and poultry products and to compare the results of full-length barcoding (658 bp) and mini-barcoding (127 bp). Sixty meat and poultry products were collected for this study, including deli meats, ground meats, dried meats, and canned meats. Each sample underwent full and mini-barcoding of the cytochrome c oxidase subunit I (COI) gene. The resulting sequences were queried against the Barcode of Life Database (BOLD) and GenBank for species identification. Overall, full-barcoding showed a higher sequencing success rate (68.3%) as compared to mini-barcoding (38.3%). Mini-barcoding out-performed full barcoding for the identification of canned products (23.8% vs. 19.0% success), as well as for turkey and duck products; however, the primer set performed poorly when tested against chicken, beef, and bison/buffalo. Overall, full barcoding was found to be a robust method for the detection of species in meat and poultry products, with the exception of canned products. Mini-barcoding shows high potential to be used for species identification in processed products; however, an improved primer set is needed for this application. (C) 2017 Elsevier Ltd. All rights reserved.