Carbon nanomaterials alter plant physiology and soil bacterial community composition in a rice-soil-bacterial ecosystem
Carbon nanomaterials alter plant physiology and soil bacterial community composition in a rice-soil-bacterial ecosystem
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碳纳米材料改变水稻-土壤-细菌生态系统中的植物生理和土壤细菌群落组成
DOI：
10.1016/j.envpol.2017.09.024
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发表时间：
2018-01-01
影响因子：
8.9
通讯作者：
Xing, Baoshan
中科院分区：
文献类型：
--
作者：
Hao, Yi;Ma, Chuanxin;Xing, Baoshan
本研究的目的是比较碳纳米材料（CNMs），即富勒烯（C-60），还原氧化石墨烯（rGO）和多壁碳纳米管（MWCNTs）的毒性效应，在一个微型生态系统的水稻种植在壤土盆栽土壤。我们测量了植物的生理生化参数和细菌群落组成的CNMS处理的植物土壤系统。30 d后，无论暴露剂量（50或500 mg/kg）如何，3种CNMs均对水稻的株高和根长产生了负面影响，根皮层细胞直径显著减小，细胞皱缩变形。此外，在高剂量CNM处理下，水稻根系中生长素、吲哚乙酸、油菜素内酯和赤霉素4的含量显著高于对照。在高剂量下，根中抗氧化酶超氧化物歧化酶（SOD）和过氧化物酶（POD）活性显著升高。高通量测序结果表明，三种典型的CNMs对土壤中优势细菌种类的迁移影响不大，但CNMs的存在显著改变了细菌群落的组成。研究结果表明，不同的CNMs对水稻和根际土壤细菌群落确实具有环境毒性，因此应合理使用CNMs本身及其掺入产物，控制其向环境中的释放/排放，以防止其对生物体的毒性效应和对食品安全的潜在风险。(C)2017爱思唯尔有限公司版权所有
The aim of this study was to compare the toxicity effects of carbon nanomaterials (CNMs), namely fullerene (C-60), reduced graphene oxide (rGO) and multi-walled carbon nanotubes (MWCNTs), on a mini ecosystem of rice grown in a loamy potted soil. We measured plant physiological and biochemical parameters and examined bacterial community composition in the CNMs-treated plant soil system. After 30 days of exposure, all the three CNMs negatively affected the shoot height and root length of rice, significantly decreased root cortical cells diameter and resulted in shrinkage and deformation of cells, regardless of exposure doses (50 or 500 mg/kg). Additionally, at the high exposure dose of CNM, the concentrations of four phytohormones, including auxin, indoleacetic acid, brassinosteroid and gibberellin acid 4 in rice roots significantly increased as compared to the control. At the high exposure dose of MWCNTs and C-60, activities of the antioxidant enzymes superoxide dismutase (SOD) and peroxidase (POD) in roots increased significantly. High-throughput sequencing showed that three typical CNMs had little effect on shifting the predominant soil bacterial species, but the presence of CNMs significantly altered the composition of the bacterial community. Our results indicate that different CNMs indeed resulted in environmental toxicity to rice and soil bacterial community in the rhizosphere and suggest that CNMs themselves and their incorporated products should be reasonably used to control their release/discharge into the environment to prevent their toxic effects on living organisms and the potential risks to food safety. (C) 2017 Elsevier Ltd. All rights reserved.