Role of intraluteal prostaglandin F2α, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum
Role of intraluteal prostaglandin F2α, progesterone and oxytocin in basal and pulsatile progesterone release from developing bovine corpus luteum
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DOI：
10.1016/j.prostaglandins.2006.01.002
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发表时间：
2006-05-01
影响因子：
2.9
通讯作者：
Skarzynski, Dariusz J.
中科院分区：
文献类型：
--
作者：
Bah, Mamadou M.;Acosta, Tomas J.;Skarzynski, Dariusz J.
本研究通过使用特定的激素拮抗剂检查黄体内前列腺素（PG）F-2α、黄体酮（P4）和催产素（OT）对黄体功能的作用。将来自发育中的 CL（动情周期第 5-7 天）的黄体细胞暴露于 P4 拮抗剂（奥那司酮，OP，10(-4) M）、OT 拮抗剂（阿托西班，AT；10(-6) M）或吲哚美辛（INDO；10-4 M）12 小时，然后用 PGF（2 α）（10（-8）M）刺激 4 小时。用 OP、AT 或 INDO 预处理细胞导致 PGF(2α) 响应的 P4 分泌增加。为了检查 P4、OT 和 PG 对 P4 分泌的时间影响，将分散的黄体细胞预先暴露于 OP、AT 或 INDO 1、2、4、6 或 12 小时。前列腺素 F-2 α。预暴露 2 小时后刺激 P4 分泌（P < 0.05）。在微透析研究中，发育中的 CL 组织自发释放的 P4 具有脉动性质，具有 1-2 It 间隔的不规则峰值。 OP 处理增加了 P4 峰的数量（P < 0.05），而 AT 和 INDO 显着减少了检测到的 P4 峰的数量（P < 0.05）。有趣的是，MO 完全阻断了 P4 释放的脉动性质，但其分泌在整个实验期间保持稳定。这些结果表明，黄体 PGF2 α、OT 和 P4 是自分泌/旁分泌卵巢内调节系统的组成部分，负责在黄体早期从牛 CL 中间歇性（脉冲式）释放 P4。 (c) 2006 Elsevier Inc. 保留所有权利。
The present study examined the role of intra-luteal prostaglandin (PG) F-2 alpha, progesterone (P4) and oxytocin (OT) on the corpus luteum function by using specific hormone antagonists. Luteal cells from the developing CL (days 5-7 of the estrous cycle) were exposed to P4 antagonist (onapristone, OP, 10(-4) M), OT antagonist (atosiban, AT; 10(-6) M) or indomethacin (INDO; 10-4 M), for 12h and then stimulated with PGF(2 alpha) (10(-8) M) for 4 h. Pre-treatment of the cells with OP, AT or INDO resulted in an increase in P4 secretion in response to PGF(2 alpha). To examine the temporal effects of P4, OT and PGs on P4 secretion, dispersed luteal cells were pre-exposed to OP, AT or INDO for 1, 2,4, 6 or 12 h. Prostaglandin F-2 alpha. stimulated P4 secretion (P < 0.05) after 2 h of pre-exposition. In the microdyalisis study, the spontaneous release of P4 from developing CL tissue was of pulsatile nature with irregular peaks at 1-2 It intervals. Treatment with OP increased the number of P4 peaks (P < 0.05), whereas AT and INDO significantly reduced the number of P4 peaks detected (P < 0.05). Interestingly, MO completely blocked the pulsatile nature in the release of P4, but it secretion remained stable troughout the experimental period. These results demonstrate that luteal PGF2 alpha, OT, and P4 are components of an autocrine/paracrine intra-ovarian regulatory system responsible for the episodic (pulsatile) release of P4 from the bovine CL during the early luteal phase. (c) 2006 Elsevier Inc. All rights reserved.