Combination of Curcumin and Metformin Inhibits Cell Growth and Induces Apoptosis without Affecting the Cell Cycle in LNCaP Prostate Cancer Cell Line
Combination of Curcumin and Metformin Inhibits Cell Growth and Induces Apoptosis without Affecting the Cell Cycle in LNCaP Prostate Cancer Cell Line
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DOI：
10.1080/01635581.2020.1783327
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发表时间：
2020-07-11
影响因子：
2.9
通讯作者：
Tarighi, Parastoo
中科院分区：
文献类型：
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作者：
Eslami, Seyed Sadegh;Jafari, Davod;Tarighi, Parastoo
副作用和化疗耐药性需要副作用最小的新疗法。在这里，我们研究了姜黄素和二甲双胍对 LNCaP 前列腺癌细胞系的联合作用。用不同浓度的姜黄素、二甲双胍及其组合处理 LNCaP 细胞。通过MTT测定评估细胞活力，并通过实时PCR分析Bax、Bcl-2、mTOR、hTERT、PUMA、p53和p21基因的表达。通过流式细胞术评估细胞凋亡和细胞周期。我们的结果表明，姜黄素、二甲双胍及其组合处理的细胞活力随着浓度的增加和处理时间的延长而显着降低（P < 0.05）。同时，该组合在48小时内显示出协同效应。在姜黄素治疗组中，Bcl-2和hTERT基因的表达减少。二甲双胍治疗组Bax和PUMA基因表达增强，而Bcl-2、hTERT、mTOR和p53基因表达下降。尽管所有治疗均诱导细胞凋亡，但姜黄素和二甲双胍的组合显示出最大水平的细胞凋亡、细胞毒性和Bax基因的表达。姜黄素和二甲双胍联合使用48小时内显示出协同效应。这种组合可能是前列腺癌的潜在治疗候选者，有待进一步研究。
Side effects and chemotherapy resistance, demand new therapeutics with minimal side effects. Here, we investigated the combined effect of curcumin and metformin on the LNCaP prostate cancer cell line. LNCaP cells were treated with curcumin, metformin, and their combination at different concentrations. Cell viability was assessed by MTT assay and expression ofBax, Bcl-2, mTOR, hTERT, PUMA, p53andp21genes was analyzed by real-time PCR. Apoptosis and cell cycle were assessed by flow cytometry. Our results revealed that the viability of cells treated with curcumin, metformin, and their combination was significantly (P < 0.05) reduced with increasing the concentration and prolonging the treatment time. Meanwhile, the combination showed a synergistic effect within 48 h. In the curcumin treated group, the expression ofBcl-2andhTERTgenes diminished. In the metformin treated group, the expression ofBaxandPUMAgenes was enhanced while the expression ofBcl-2,hTERT,mTOR, andp53genes declined. Although all treatments induced apoptosis, the combination of curcumin and metformin showed the maximum level of apoptosis, cytotoxicity, and expression ofBaxgene. The combination of curcumin and metformin showed synergistic effects within 48 h. This combination could be a potential therapeutic candidate for prostate cancer to be further investigated.