Spherulocytes in the echinoderm Holothuria glaberrima and their involvement in intestinal regeneration
Spherulocytes in the echinoderm Holothuria glaberrima and their involvement in intestinal regeneration
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DOI：
10.1002/dvdy.20983
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发表时间：
2006-12-01
影响因子：
2.5
通讯作者：
Candelaria, Ann Ginette
中科院分区：
文献类型：
--
作者：
Garcia-Arraras, Jose E.;Schenk, Christian;Candelaria, Ann Ginette
光斑Holothuria glaberrima在内脏取出后可以再生其肠道。含球形细胞，即球形细胞，似乎与肠道再生有关。我们使用组织化学和免疫细胞化学来表征这些细胞及其在再生过程中的作用。球形细胞直径为10-20 μ m，细胞核为单中心，细胞质内有球状结构。它们存在于未切除和再生动物的肠和肠系膜的结缔组织中。在再生的第二周，再生肠内的球细胞数量增加，形态发生显著变化。在形态学改变的同时，细胞的免疫组织化学标记也发生了变化；抗体不仅能识别小球结构，还能以更均匀的模式标记细胞质。此外，免疫组织化学标记似乎也分散在细胞外基质中，表明细胞正在释放其囊泡内容物。球型红细胞也存在于其他组织中，通常与结缔组织成分有关。我们的数据强烈提示球型红细胞参与肠道再生，但其具体作用仍不确定。总之，我们的数据扩展了我们对棘皮动物中与再生过程相关的细胞事件的认识，并提供了与脊椎动物中类似过程的比较。
The holothuroid echinoderm Holothuria glaberrima can regenerate its intestine after a process of evisceration. Spherule-containing cells, the spherulocytes, appear to be associated with intestinal regeneration. We have used histochemistry and immunocytochemistry to characterize these cells and their role in the regeneration process. Spherulocytes are 10-20 mu m in diameter with an acrocentric nucleus and spherule-like structures within their cytoplasm. They are found in the connective tissue of the intestine and mesentery of noneviscerated and regenerating animals. During the second week of regeneration, the number of spherulocytes in the regenerating intestine increases and a dramatic change in their morphology occurs. Together with the morphological change, the immunohistochemical labeling of the cells also changes; the antibodies not only recognize the spherule structures but also label the cellular cytoplasm in a more homogeneous pattern. Moreover, immunohistochemical labeling also appears to be dispersed within the extracellular matrix, suggesting that the cells are liberating their vesicular contents. Spherulocytes are found in other tissues of H. glaberrima, always associated with the connective tissue component. Our data strongly suggest that spherulocytes are involved in intestinal regeneration but their specific role remains undetermined. In summary, our data expand our knowledge of the cellular events associated with regeneration processes in echinoderms and provide for comparisons with similar processes in vertebrates.