INFECTION OF THE SCID-HU MOUSE BY HIV-1
INFECTION OF THE SCID-HU MOUSE BY HIV-1
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DOI：
10.1126/science.3201256
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发表时间：
1988-12-23
期刊：
影响因子：
56.9
通讯作者：
MCCUNE, JM
中科院分区：
文献类型：
--
作者：
NAMIKAWA, R;KANESHIMA, H;MCCUNE, JM
用克隆的人免疫缺陷病毒-1分离株HIV-1 JR-CSF接种带有人胎儿胸腺或淋巴结移植物的SCID-hu小鼠。以时间和剂量依赖的方式，病毒复制在人类淋巴器官内传播。结合免疫组化和原位杂交显示，在大多数感染的细胞中只有病毒RNA转录本，但有些细胞既有可检测到的病毒转录本和病毒蛋白。感染细胞在胸腺髓质中总是比在皮质中更明显。这些研究表明，在SCID-hu小鼠中，可以观察到HIV-1对人类淋巴器官的急性感染。
SCID-hu mice with human fetal thymic or lymph node implants were inoculated with the cloned human immunodeficiency virus-1 isolate, HIV-1JR-CSF. In a time- and dose-dependent fashion, viral replication spread within the human lymphoid organs. Combination immunohistochemistry and in situ hybridization revealed only viral RNA transcripts in most infected cells, but some cells had both detectable viral transcripts and viral protein. Infected cells were always more apparent in the medulla than in the cortex of the thymus. These studies demonstrate that an acute infection human lymphoid organs with HIV-1 can be followed in the SCID-hu mouse.